Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE
文献类型：期刊论文
| 作者 | Hu Qing; Dou Min-na; Qi Hong-yan; Xe Xiang-ming; Zhuang Guo-qiang; Yang Min |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2007 |
| 卷号 | 19期号:9页码:1114-1119 |
| 关键词 | Bacillus Cereus Enterobacter Cloacae Pcr-dgge Cadmium Resistance Antibiotic Resistance |
| 英文摘要 | This study focused on the screening of cadmium-resistant bacterial strains from Pb-Zn tailing. We investigated the diversity of microbial community inhabiting Dong-san-cha Pb-Zn tailing in Beijing, China, by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene of bacterial strain, and found two dominant strains in the DGGE profile. Using special culture media, we isolated two strong cadmium-resistant bacterial strains. On the basis of morphological, physiological, and biochemical characteristics, BIOLOG, and 16S rDNA sequencing, the two strains were identified as Bacillus cereus and Enterobacter cloacae. Minimal inhibitory concentrations (MICs) of heavy metals for the bacteria were determined. E. cloacae showed higher MIC values for heavy metals and a larger range of antibiotic resistance than B. cereus. |
| WOS记录号 | WOS:000249573100016 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22666]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Hu Qing,Dou Min-na,Qi Hong-yan,et al. Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2007,19(9):1114-1119. |
| APA | Hu Qing,Dou Min-na,Qi Hong-yan,Xe Xiang-ming,Zhuang Guo-qiang,&Yang Min.(2007).Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,19(9),1114-1119. |
| MLA | Hu Qing,et al."Detection, isolation, and identification of cadmium-resistant bacteria based on PCR-DGGE".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 19.9(2007):1114-1119. |
入库方式： OAI收割
来源：生态环境研究中心
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