Isolation of levoglucosan-assimilating microorganisms from soil and an investigation of their levoglucosan kinases
文献类型：期刊论文
| 作者 | Xie, Huijun; Zhuang, Xuliang; Bai, Zhihui; Qi, Hongyan; Zhang, Hongxun |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2006 |
| 卷号 | 22期号:9页码:887-892 |
| 关键词 | Levoglucosan Kinase 1 Levoglucosan-assimilating Organisms 6-anhydro-beta-d-glucose Protein Property Protein Purification Soil |
| 英文摘要 | The phosphorylation activities of glucose and levoglucosan (1,6-anhydro-beta-D-glucopyranose, LG) were studied in 26 types of LG-assimilating microorganisms isolated from four types of soil in China. The results showed that activities of LG kinase production in most of the yeasts were lower than those of filamentous fungi, and that the highest filamentous fungal activities (0.61 U/mg protein) was approximately twice that of yeast. The filamentous fungi and yeast with highest LG kinase production activity were identified by DNA sequence analysis of 18S or 26S rRNA gene fragments respectively. The identities of them were Alternaria alternata, Eupenicillium javanicum, Aspergillus niger, Penicillium herquei, Cryptococcus laurentii, Cryptococcus flavescens, Cryptococcus luteolus and Rhodotorula aurantiaca, respectively. In addition, the LG kinases from both types of organisms were purified. The effects of temperature and pH on LG kinase activity were also studied. There was very little change in activity between pH 7 to 10 and at temperatures below 30 degrees C. The apparent K-m values for ATP were also similar in different microorganisms, but K-m values for LG ranged from 48 to 102 mM. |
| WOS记录号 | WOS:000241361400001 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22814]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Xie, Huijun,Zhuang, Xuliang,Bai, Zhihui,et al. Isolation of levoglucosan-assimilating microorganisms from soil and an investigation of their levoglucosan kinases[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2006,22(9):887-892. |
| APA | Xie, Huijun,Zhuang, Xuliang,Bai, Zhihui,Qi, Hongyan,&Zhang, Hongxun.(2006).Isolation of levoglucosan-assimilating microorganisms from soil and an investigation of their levoglucosan kinases.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,22(9),887-892. |
| MLA | Xie, Huijun,et al."Isolation of levoglucosan-assimilating microorganisms from soil and an investigation of their levoglucosan kinases".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 22.9(2006):887-892. |
入库方式： OAI收割
来源：生态环境研究中心
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