Screening and identification of the levoglucosan kinase gene (lgk) from Aspergillus niger by LC-ESI-MS/MS and RT-PCR
文献类型：期刊论文
| 作者 | Xie, HJ; Zhuang, XA; Zhang, HX; Bai, ZH; Qi, HY |
| 刊名 | FEMS MICROBIOLOGY LETTERS
 |
| 出版日期 | 2005 |
| 卷号 | 251期号:2页码:313-319 |
| 关键词 | Lgk Gene Levoglucosan Kinase Lc-esi-ms/ms Rt-pcr Gene Cloning And Expression |
| DOI | 10.1016/j.femsie.2005.08.024 |
| 英文摘要 | A protein of 75,000 Daltons with levoglucosan kinase activity was purified from Aspergillus niger. After in-gel digestion by trypsin, a 14-mer peptide was sequenced and analyzed by LC-ESI-MS/MS. Using a primer derived from the 14-mer peptide in combination with Oligo-(dT)(18), a cDNA fragment was obtained by RT-PCR. A search of the GenBank database indicated that the protein had not been identified before. A similar protein named hypothetical protein FG07802.1 (EAA77996.1) was found to exist in Gibberella zeae by Blastx search. Using a primer derived from the protein, a cDNA fragment of second RT-PCR was cloned into plasmid pAJ401, which was transformed to Saccharomyces cerevisiae H158 and expressed. Two positive levoglucosan assimilating recombinants were selected. The lgk gene was screened and identified. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Microbiology |
| WOS研究方向 | Microbiology |
| WOS记录号 | WOS:000232437800018 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23373]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Xie, HJ,Zhuang, XA,Zhang, HX,et al. Screening and identification of the levoglucosan kinase gene (lgk) from Aspergillus niger by LC-ESI-MS/MS and RT-PCR[J]. FEMS MICROBIOLOGY LETTERS,2005,251(2):313-319. |
| APA | Xie, HJ,Zhuang, XA,Zhang, HX,Bai, ZH,&Qi, HY.(2005).Screening and identification of the levoglucosan kinase gene (lgk) from Aspergillus niger by LC-ESI-MS/MS and RT-PCR.FEMS MICROBIOLOGY LETTERS,251(2),313-319. |
| MLA | Xie, HJ,et al."Screening and identification of the levoglucosan kinase gene (lgk) from Aspergillus niger by LC-ESI-MS/MS and RT-PCR".FEMS MICROBIOLOGY LETTERS 251.2(2005):313-319. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。