Assessment of the bacterial diversity in fenvalerate-treated soil
文献类型：期刊论文
| 作者 | Luo, HF; Qi, HY; Zhang, HX |
| 刊名 | WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
 |
| 出版日期 | 2004 |
| 卷号 | 20期号:5页码:509-515 |
| 关键词 | Bacterial Community Colony Morphology Dgge Diversity Fenvalerate |
| DOI | 10.1023/B:WIBI.0000040401.46606.a4 |
| 英文摘要 | The impact of the pesticide fenvalerate on the diversity of the bacterial community in soil was investigated in this study. After treatment with 0.1, 0.5 or 1.0 mg fenvalerate g(-1) soil in three soils and incubation for a 40-day period, the changes in diversity were monitored by two different methods. The cultivable heterotrophic diversity was investigated by colony morphology on solid LB medium. Genetic diversity was measured as bands on denaturing gradient gel electrophoresis (DGGE) gels by total genomic DNA extraction and purification, PCR-amplification of bacterial 16S rDNA fragments. The Shannon-Wiener index of diversity (H), richness (S) and evenness (EH) were used to measure changes in the bacterial community in the soils. The results of the cultivable heterotrophic diversity and genetic diversity showed that there was an obvious decrease in diversity due to the application of fenvalerate to the soils, and the different amounts added had different impacts on the diversity. Bands appearing to be either enhanced or inhibited as a result of the fenvalerate treatments were excized and sequenced. Sequencing of excized DGGE bands indicated that application of fenvalerate had an obvious impact on several Pseudomonas spp., or Xanthomonas campestris or Streptomyces avermitilis. This revealed that microbial community changes can occur due to the application of fenvalerate to soil. |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000223741700012 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23583]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Luo, HF,Qi, HY,Zhang, HX. Assessment of the bacterial diversity in fenvalerate-treated soil[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2004,20(5):509-515. |
| APA | Luo, HF,Qi, HY,&Zhang, HX.(2004).Assessment of the bacterial diversity in fenvalerate-treated soil.WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,20(5),509-515. |
| MLA | Luo, HF,et al."Assessment of the bacterial diversity in fenvalerate-treated soil".WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 20.5(2004):509-515. |
入库方式： OAI收割
来源：生态环境研究中心
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