Diversity surveys of soil bacterial community by cultivation-based methods and molecular fingerprinting techniques
文献类型：期刊论文
| 作者 | Luo, HF; Qi, HY; Zhang, HX |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2004 |
| 卷号 | 16期号:4页码:581-584 |
| 关键词 | Bacterial Community Colony Morphology Dgge Diversity |
| 英文摘要 | By combining the cultivation methods with molecular fingerprinting techniques, the diversity surveys of soil bacterial community in 13 areas of China were carried out. The cultivable heterotrophic diversity was investigated by colony morphology on solid LB medium. Genetic diversity was measured as bands on denaturing gradient gel electrophoresis(DGGE) by the extraction and purification of the total soil DNA, and amplification of bacterial 16S rDNA fragments by polymerase chain reaction(PCR). The Shannon-Wiener indices of diversity(H), richness(S) and evenness(E-H) were employed to estimate the diversity of soil bacterial community. The results showed that there was an obvious diversification existed in soil from the different areas. However, the genetic diversity estimated by PCR-DGGE can provide more comprehensive information on bacterial community than the cultivation-based methods. Therefore, it is suggested to combine the traditional methods with genetic fingerprinting techniques to survey and estimate soil bacterial diversity. |
| WOS研究方向 | Environmental Sciences & Ecology |
| WOS记录号 | WOS:000222450600012 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23587]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Luo, HF,Qi, HY,Zhang, HX. Diversity surveys of soil bacterial community by cultivation-based methods and molecular fingerprinting techniques[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2004,16(4):581-584. |
| APA | Luo, HF,Qi, HY,&Zhang, HX.(2004).Diversity surveys of soil bacterial community by cultivation-based methods and molecular fingerprinting techniques.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,16(4),581-584. |
| MLA | Luo, HF,et al."Diversity surveys of soil bacterial community by cultivation-based methods and molecular fingerprinting techniques".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 16.4(2004):581-584. |
入库方式： OAI收割
来源：生态环境研究中心
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