A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus
文献类型：期刊论文
| 作者 | Zhang, Yinfeng; Li, Yuanyuan; Qin, Zhanfen; Wang, Huili; Li, Jianzhong |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 34页码:143-154 |
| 关键词 | Thyroid Hormone Signaling Disruptor Pelophylax Nigromaculatus Th-response Gene Expression Screening Assay Tetrabromobisphenol a |
| 英文摘要 | Amphibian metamorphosis provides a wonderful model to study the thyroid hormone (TH) signaling disrupting activity of environmental chemicals, with Xenopus laevis as the most commonly used species. This study aimed to establish a rapid and sensitive screening assay based on TH-response gene expression analysis using Pelophylax nigromaculatus, a native frog species distributed widely in East Asia, especially in China. To achieve this, five candidate TH-response genes that were sensitive to T3 induction were chosen as molecular markers, and T3 induction was determined as 0.2 nmol/L T3 exposure for 48 hr. The developed assay can detect the agonistic activity of T3 with a lowest observed effective concentration of 0.001 nmol/L and EC50 at around 0.118-1.229 nmol/L, exhibiting comparable or higher sensitivity than previously reported assays. We further validated the efficiency of the developed assay by detecting the TH signaling disrupting activity of tetrabromobisphenol A (TBBPA), a known TH signaling disruptor. In accordance with previous reports, we found a weak TH agonistic activity for TBBPA in the absence of T3, whereas a TH antagonistic activity was found for TBBPA at higher concentrations in the presence of T3, showing that the P. nigromaculatus assay is effective for detecting TH signaling disrupting activity. Importantly, we observed non-monotonic dose-dependent disrupting activity of TBBPA in the presence of T3, which is difficult to detect with in vitro reporter gene assays. Overall, the developed P. nigromaculatus assay can be used to screen TH signaling disrupting activity of environmental chemicals with high sensitivity. (C) 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| WOS研究方向 | Environmental Sciences |
| WOS记录号 | WOS:000362980400016 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32908]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Yinfeng,Li, Yuanyuan,Qin, Zhanfen,et al. A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2015,34:143-154. |
| APA | Zhang, Yinfeng,Li, Yuanyuan,Qin, Zhanfen,Wang, Huili,&Li, Jianzhong.(2015).A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus.JOURNAL OF ENVIRONMENTAL SCIENCES,34,143-154. |
| MLA | Zhang, Yinfeng,et al."A screening assay for thyroid hormone signaling disruption based on thyroid hormone-response gene expression analysis in the frog Pelophylax nigromaculatus".JOURNAL OF ENVIRONMENTAL SCIENCES 34(2015):143-154. |
入库方式： OAI收割
来源：生态环境研究中心
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