Detection of bioavailable cadmium, lead, and arsenic in polluted soil by tailored multiple Escherichia coli whole-cell sensor set
文献类型：期刊论文
| 作者 | Hou, Qihui; Ma, Anzhou; Wang, Thanh; Lin, Jianqiang; Wang, Hailin; Du, Binghai; Zhuang, Xuliang; Zhuang, Guoqiang |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2015-08 |
| 卷号 | 407期号:22页码:6865-6871 |
| 关键词 | Heavy Metals Bioavailability Specificity Whole-cell Sensor Set Cross-mixed Induction Binary Linear Regression |
| 英文摘要 | Microbial whole-cell sensor has been widely used to assess bioavailability and risk of toxic elements, but their environmental use is still limited due to the presence of other interfering pollutants and the nonspecific binding in cells, which leads to inaccurate results. Here, we proposed a strategy combining Escherichia coli sensor set with binary regression models for the specific detection of bioavailable cadmium (Cd), lead (Pb), and arsenic (As) in a co-polluted environment. Initial tests suggested that the sensor set respectively termed pcadCluc, pzntRluc, and parsRluc could be classified into two groups according to their specific response to Cd, Pb, and As: group 1 (pcadCluc and pzntRluc) induced by a Cd-Pb mix and group 2 (parsRluc) induced by a Cd-As mix. Based on the variance in responses of each sensor to mixtures of target elements, three binary linear equations for two sensor groups were set up to calculate the individual concentrations in the mixture solutions. This method was then used to quantify the bioavailable Cd, Pb, and As in soils from a co-polluted mining region and to compare the results with other methods. Results showed that the conventional single target sensor method overestimated the bioavailability of each element, while sensor set was credible for accurate bioavailable Cd, Pb, and As quantification and comparable with the results from inductively coupled plasma mass spectrometry (ICP-MS) analysis. Our method can potentially be extended to cover the specific detection of other bioavailable toxic elements in different environmental settings. |
| WOS研究方向 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000360220800028 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/32920]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Hou, Qihui,Ma, Anzhou,Wang, Thanh,et al. Detection of bioavailable cadmium, lead, and arsenic in polluted soil by tailored multiple Escherichia coli whole-cell sensor set[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2015,407(22):6865-6871. |
| APA | Hou, Qihui.,Ma, Anzhou.,Wang, Thanh.,Lin, Jianqiang.,Wang, Hailin.,...&Zhuang, Guoqiang.(2015).Detection of bioavailable cadmium, lead, and arsenic in polluted soil by tailored multiple Escherichia coli whole-cell sensor set.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,407(22),6865-6871. |
| MLA | Hou, Qihui,et al."Detection of bioavailable cadmium, lead, and arsenic in polluted soil by tailored multiple Escherichia coli whole-cell sensor set".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 407.22(2015):6865-6871. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。