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Autotrophic ammonia oxidation by soil thaumarchaea
文献类型：期刊论文
| 作者 | Zhang, Li-Mei; Offre, Pierre R.; He, Ji-Zheng; Verhamme, Daniel T.; Nicol, Graeme W.; Prosser, James I. |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2010-10-05 |
| 卷号 | 107期号:40页码:17240-17245 |
| 关键词 | Ammonia Oxidizers Nitrification Soil Autotrophy Stable Isotope Probing |
| 英文摘要 | Nitrification plays a central role in the global nitrogen cycle and is responsible for significant losses of nitrogen fertilizer, atmospheric pollution by the greenhouse gas nitrous oxide, and nitrate pollution of groundwaters. Ammonia oxidation, the first step in nitrification, was thought to be performed by autotrophic bacteria until the recent discovery of archaeal ammonia oxidizers. Autotrophic archaeal ammonia oxidizers have been cultivated from marine and thermal spring environments, but the relative importance of bacteria and archaea in soil nitrification is unclear and it is believed that soil archaeal ammonia oxidizers may use organic carbon, rather than growing autotrophically. In this soil microcosm study, stable isotope probing was used to demonstrate incorporation of (13)C-enriched carbon dioxide into the genomes of thaumarchaea possessing two functional genes: amoA, encoding a subunit of ammonia monooxygenase that catalyses the first step in ammonia oxidation; and hcd, a key gene in the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle, which has been found so far only in archaea. Nitrification was accompanied by increases in archaeal amoA gene abundance and changes in amoA gene diversity, but no change was observed in bacterial amoA genes. Archaeal, but not bacterial, amoA genes were also detected in (13)C-labeled DNA, demonstrating inorganic CO(2) fixation by archaeal, but not bacterial, ammonia oxidizers. Autotrophic archaeal ammonia oxidation was further supported by coordinate increases in amoA and hcd gene abundance in (13)C-labeled DNA. The results therefore provide direct evidence for a role for archaea in soil ammonia oxidation and demonstrate autotrophic growth of ammonia oxidizing archaea in soil. |
| WOS记录号 | WOS:000282512000036 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/21322]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Li-Mei,Offre, Pierre R.,He, Ji-Zheng,et al. Autotrophic ammonia oxidation by soil thaumarchaea[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2010,107(40):17240-17245. |
| APA | Zhang, Li-Mei,Offre, Pierre R.,He, Ji-Zheng,Verhamme, Daniel T.,Nicol, Graeme W.,&Prosser, James I..(2010).Autotrophic ammonia oxidation by soil thaumarchaea.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,107(40),17240-17245. |
| MLA | Zhang, Li-Mei,et al."Autotrophic ammonia oxidation by soil thaumarchaea".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 107.40(2010):17240-17245. |
入库方式： OAI收割
来源：生态环境研究中心
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