In vivo and in vitro anti-hepatitis B virus activity of total phenolics from Oenanthe javanica
文献类型：期刊论文
| 作者 | Han, Yan-Quan; Wu, Guang-Xia; Liu, He-Zhi; Yang, Xin-Bo; Huang, Zheng-Ming |
| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2008-06-19 |
| 卷号 | 118期号:1页码:148-153 |
| 关键词 | Oenanthe Javanica Phenolics Anti-hbv Activity Hep G2.2.15 Cells Duck Hepatitis b Virus |
| 英文摘要 | The traditional Chinese medicine Oenanthe javanica (OJ) has been used for many years, mainly for the treatment of inflammatory conditions including hepatitis. In this study, human hepatoma Hep G2.2.15 cells culture system and duck hepatitis B virus (DHBV) infection model were used as in vivo and in vitro models to evaluate the anti-HBV effects of total phenolics from Oenanthe javanica (OJTP). The HBeAg and HBsAg concentrations in cell culture medium were determined by using the enzyme immunoassay kit after Hep G2.2.15 cells were treated with OJTP for 9d. DHBV-DNA in duck serum was analyzed by dot blot hybridization assay. In the cell model, OJTP could dose-dependently inhibit the production of the HBeAg and HBsAg, and the inhibition rates of OJTP on HBeAg and HBsAg in the Hep G2.2.15 cells were 70.12% and 72.61% on day 9, respectively. In the DHBV infection model, OJTP also reduced HBV DNA level in a dose-dependent manner. The DHBV-DNA levels decreased significantly after the treatment with 0.10 g kg(-1) d(-1) and 0.20 g kg(-1) d(-1) OJTP. The inhibition of the peak of viremia was at the maximum at the dose of 0.20 g kg(-1) d(-1) and reached 64.10% on day 5 and 66.48% on day 10, respectively. Histopathological evaluation of the liver revealed significant improvement by OJTP. In conclusion, our results demonstrate that OJTP can efficiently inhibit HBV replication in Hep G2.2.15 cells line in vitro and inhibit DHBV replication in ducks in vivo. OJTP therefore warrants further investigation as a potential therapeutic agent for HBV infections. (C) 2008 Elsevier Ireland Ltd. All rights reserved. |
| WOS记录号 | WOS:000257335600022 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22299]  |
| 专题 | 生态环境研究中心_水污染控制实验室 |
| 推荐引用方式 GB/T 7714 | Han, Yan-Quan,Wu, Guang-Xia,Liu, He-Zhi,et al. In vivo and in vitro anti-hepatitis B virus activity of total phenolics from Oenanthe javanica[J]. JOURNAL OF ETHNOPHARMACOLOGY,2008,118(1):148-153. |
| APA | Han, Yan-Quan,Wu, Guang-Xia,Liu, He-Zhi,Yang, Xin-Bo,&Huang, Zheng-Ming.(2008).In vivo and in vitro anti-hepatitis B virus activity of total phenolics from Oenanthe javanica.JOURNAL OF ETHNOPHARMACOLOGY,118(1),148-153. |
| MLA | Han, Yan-Quan,et al."In vivo and in vitro anti-hepatitis B virus activity of total phenolics from Oenanthe javanica".JOURNAL OF ETHNOPHARMACOLOGY 118.1(2008):148-153. |
入库方式： OAI收割
来源：生态环境研究中心
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