A CDC25 homologue from rice functions as an arsenate reductase
文献类型：期刊论文
| 作者 | Tong, Yi-Ping; Mukhopadhyay, Rita; Duan, Gui-Lan; Zhou, Yao; Rosen, Barry P.; Zhu, Yong-Guan |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2007 |
| 卷号 | 174期号:2页码:311-321 |
| 关键词 | Arsenate Reductase Arsenic Speciation Cdc25 (Cell Division Cycle) Metabolism Rice (Oryza Sativa) Transcriptional Patterns |
| 英文摘要 | Enzymatic reduction of arsenate to arsenite is the first step in arsenate metabolism in all organisms studied. The rice genome contains two ACR2-like genes, OsACR2.1 and OsACR2.2, which may be involved in regulating arsenic metabolism in rice. Here, we cloned both OsACR2 genes and expressed them in an Escherichia coli strain in which the arsC gene was deleted and in a yeast (Saccharomyces cerevisiae) strain with a disrupted ACR2 gene. OsACR2.1 complemented the arsenate hypersensitive phenotype of E. coli and yeast. OsACR2.2 showed much less ability to complement. The gene products were purified and demonstrated to reduce arsenate to arsenite in vitro, and both exhibited phosphatase activity. In agreement with the complementation results, OsACR2.1 exhibited higher reductase activity than OsACR2.2. Mutagenesis of cysteine residues in the putative active site HC(X)(5)R motif led to nearly complete loss of both phosphatase and arsenate reductase activities. In planta expression of OsACR2.1 increased dramatically after exposure to arsenate. OsACR2.2 was observed only in roots following arsenate exposure, and its expression was less than OsACR2.1. |
| WOS记录号 | WOS:000245413900014 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/22623]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Tong, Yi-Ping,Mukhopadhyay, Rita,Duan, Gui-Lan,et al. A CDC25 homologue from rice functions as an arsenate reductase[J]. NEW PHYTOLOGIST,2007,174(2):311-321. |
| APA | Tong, Yi-Ping,Mukhopadhyay, Rita,Duan, Gui-Lan,Zhou, Yao,Rosen, Barry P.,&Zhu, Yong-Guan.(2007).A CDC25 homologue from rice functions as an arsenate reductase.NEW PHYTOLOGIST,174(2),311-321. |
| MLA | Tong, Yi-Ping,et al."A CDC25 homologue from rice functions as an arsenate reductase".NEW PHYTOLOGIST 174.2(2007):311-321. |
入库方式： OAI收割
来源：生态环境研究中心
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