Analyses of soil fungal communities in adjacent natural forest and hoop pine plantation ecosystems of subtropical Australia using molecular approaches based on 18S rRNA genes
文献类型：期刊论文
| 作者 | Xu, ZH; Hughes, J; He, JZ |
| 刊名 | FEMS MICROBIOLOGY LETTERS
 |
| 出版日期 | 2005 |
| 卷号 | 247期号:1页码:91-100 |
| 关键词 | Soil Fungal Community 18s Rrna Gene Cloning Tgge Sscp |
| DOI | 10.1016/j.femsle.2005.04.033 |
| 英文摘要 | Soil fungal communities were studied using 18S rDNA-based molecular techniques. Soil DNA was analyzed using temperature gradient gel electrophoresis (TGGE), single-stranded conformational polymorphism (SSCP), cloning and sequencing methods, following community DNA extraction and polymerase chain reaction (PCR). The extracted community DNA was successfully amplified using the primer pair of EF4f-Fung5r which produced ca. 550 bp 18S rDNA fragments. TGGE screening of the PCR products showed some differences in band position and intensity between two soil samples in adjacent natural forest (YNF) and hoop pine plantation (YHP) ecosystems at Yarraman in subtropical Australia. TGGE and SSCP could be used for screening PCR products. However, care must be exercised when interpreting the TGGE and SSCP results with respect to microbial diversity, because one band may not necessarily represent one species. It is recommended that the PCR products should be purified before TGGE or SSCP screening. SSCP screening of the clone sequences revealed differences among the clones. Sequence and phylogenetic analyses revealed that all obtained clones were affiliated to the kingdom Fungi, including three phyla, i.e., Zygomycota, Ascomycota and Basidiomycota. Our results suggested that community DNA extraction, PCR, cloning, SSCP screening of clones, sequencing of selected clones and phylogentic analyses could be a good strategy in investigation of soil fungal community and diversity. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. |
| 学科主题 | Microbiology |
| WOS研究方向 | Microbiology |
| WOS记录号 | WOS:000229707200012 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23330]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Xu, ZH,Hughes, J,He, JZ. Analyses of soil fungal communities in adjacent natural forest and hoop pine plantation ecosystems of subtropical Australia using molecular approaches based on 18S rRNA genes[J]. FEMS MICROBIOLOGY LETTERS,2005,247(1):91-100. |
| APA | Xu, ZH,Hughes, J,&He, JZ.(2005).Analyses of soil fungal communities in adjacent natural forest and hoop pine plantation ecosystems of subtropical Australia using molecular approaches based on 18S rRNA genes.FEMS MICROBIOLOGY LETTERS,247(1),91-100. |
| MLA | Xu, ZH,et al."Analyses of soil fungal communities in adjacent natural forest and hoop pine plantation ecosystems of subtropical Australia using molecular approaches based on 18S rRNA genes".FEMS MICROBIOLOGY LETTERS 247.1(2005):91-100. |
入库方式： OAI收割
来源：生态环境研究中心
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