Molecular cloning and characterization of a DFR from developing seeds of blue-grained wheat in anthocyanin biosynthetic pathway
文献类型：期刊论文
| 作者 | Yang, GH; Tong, YP; Li, ZS; Zheng, Q; Liu, JZ; Li, B; Zhao, XQ |
| 刊名 | ACTA BOTANICA SINICA
 |
| 出版日期 | 2003 |
| 卷号 | 45期号:11页码:1329-1338 |
| 关键词 | Blue-grained Wheat Anthocyanin Biosynthetic Pathway Dihydroflavonol 4-reductase (Dfr) Rapid Amplification Of Cdna Ends (Race) Thinopyrum Ponticum (2n=70) |
| 英文摘要 | Blue-grained wheat derived from the hybrid Triticum aestivum L. X Thinopyrum ponticum (Podp.) Barkworth et D. R. Dewey (Agropyron elongatum (Host) P. Beauv., 2n=70). The molecular biological mechanism of the biosynthetic pathway of blue pigments in the blue grain remains unclear yet. Dihydroflavonol 4-reductase (DFR) is one of the key enzymes controlling flavonoid synthesis in anthocyanin biosynthetic pathway, and may directly participate in the formation of blue pigment in the aleurone layer of blue-grained wheat. Here we cloned a DFR cDNA (TaDFR) from the developing seeds of blue-grained wheat, and four DFR genomic DNAs from Th. ponticum (ThpDFR.t), blue-grained wheat (TaDFR.bg), white-grained offspring of light blue-grained wheat (TaDFR.wg) and Chinese Spring (2n=42) (TaDFR.csg), respectively. TaDFR cDNA encodes a 354 amino-acids polypeptide with high identity to DFR from Hordeum vulgare L. (94%), Oryza sativa L. (83%), Zea mays L.(84%). The result of cluster analysis showed that TaDFR cDNA nucleotide sequence has 100% identity with that of TaDFR.csg. The four DFR genomic DNAs have extraordinary high homology and each has three introns. The differences of the four DFR genomic DNAs mainly exist in introns. Southern blotting analysis showed that there are at least 3-5 DFR copies in wheat, the copy numbers in different color grain wheats are not significantly different. The hybridization band patterns were the same, but different from that of Th. ponticum. DFR in blue-grained wheat belongs to a DFR superfamily. Northern blotting analysis indicated that the DFR expressed in the developing seeds of both blue- and white-grained wheat at 15 d after flowering (DAF), the mRNA levels of DFR reached the highest at 18 DAF, then declined quickly and disappeared at 33 DAF But the expression levels in blue-grained seeds were higher than that in white grain at the same seed developing stages. DFR transcripts accumulated in young leaves, and leaf sheaths of blue- and white-grained wheat and Th ponticum, but not detected in roots from different color wheats and developing seeds of Th. ponticum. Results indicated that there may exist some regulatory gene(s) which can increase the expression of DFR in the aleurone layer of blue-grained wheat, and thus resulting in the formation of blue pigments. |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS记录号 | WOS:000186602800011 |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/23803]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Yang, GH,Tong, YP,Li, ZS,et al. Molecular cloning and characterization of a DFR from developing seeds of blue-grained wheat in anthocyanin biosynthetic pathway[J]. ACTA BOTANICA SINICA,2003,45(11):1329-1338. |
| APA | Yang, GH.,Tong, YP.,Li, ZS.,Zheng, Q.,Liu, JZ.,...&Zhao, XQ.(2003).Molecular cloning and characterization of a DFR from developing seeds of blue-grained wheat in anthocyanin biosynthetic pathway.ACTA BOTANICA SINICA,45(11),1329-1338. |
| MLA | Yang, GH,et al."Molecular cloning and characterization of a DFR from developing seeds of blue-grained wheat in anthocyanin biosynthetic pathway".ACTA BOTANICA SINICA 45.11(2003):1329-1338. |
入库方式： OAI收割
来源：生态环境研究中心
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