Augmentation of protein-derived acetic acid production by heat-alkaline-induced changes in protein structure and conformation
文献类型：期刊论文
| 作者 | Qu, Jiuhui; Li, Yanbo; Liu, Junxin; Ren, Nan-Qi; Wang, Xu |
| 刊名 | WATER RESEARCH
 |
| 出版日期 | 2016-01 |
| 卷号 | 88页码:595-603 |
| 关键词 | Waste Activated Sludge Fermentation Pretreatment Acetic Acid Protein Structure And Conformation Economic Analysis |
| DOI | 10.1016/j.watres.2015.10.055 |
| 英文摘要 | Waste-derived acetic acid (HAc) is an attractive feedstock for microbe-mediated biofuel production. However, fermentative conversion of HAc from waste-activated sludge (WAS) has low yield because of the high concentration of proteins not readily utilizable by microorganisms without prior hydrolysis. We investigated a combined technology for HAc augmentation during sludge protein fermentation. The maximal HAc yield increased over two-fold, reaching 0.502 +/- 0.021 g/g protein (0.36 +/- 0.01 g COD/g COD, similar to 52% of the total volatile fatty acids) when synthetic sludge protein was heated at 120 degrees C for 30 min, treated at pH 12 for 24 h, and fermented at pH 9 for 72 h. Comprehensive analysis illustrated that the heat-alkaline pretreatment significantly induced protein fragmentation, simultaneously increasing the efficiency of protein biohydrolysis (from 35.5% to 85.9%) by inducing conformational changes indicative of protein unfolding. Consequently, the native alpha-helix content was decreased from 67.3% to 32.5% by conversion to an unordered shape, whose content increased from 27.5% to 45.5%; disulfide bonds were cleaved, whereas the main S-S stretching pattern was altered from gauche-gauche-gauche to gauche-gauche-trans, consequently causing increased protein susceptibility to proteolytic hydrolysis (76.3% vs. 47.0%). Economic analysis indicated that anaerobic fermentation with appropriate heat-alkaline pretreatment is a cost-effective approach for waste conversion to energy sources such as HAc. (C) 2015 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/36228]  |
| 专题 | 生态环境研究中心_水污染控制实验室 |
| 推荐引用方式 GB/T 7714 | Qu, Jiuhui,Li, Yanbo,Liu, Junxin,et al. Augmentation of protein-derived acetic acid production by heat-alkaline-induced changes in protein structure and conformation[J]. WATER RESEARCH,2016,88:595-603. |
| APA | Qu, Jiuhui,Li, Yanbo,Liu, Junxin,Ren, Nan-Qi,&Wang, Xu.(2016).Augmentation of protein-derived acetic acid production by heat-alkaline-induced changes in protein structure and conformation.WATER RESEARCH,88,595-603. |
| MLA | Qu, Jiuhui,et al."Augmentation of protein-derived acetic acid production by heat-alkaline-induced changes in protein structure and conformation".WATER RESEARCH 88(2016):595-603. |
入库方式： OAI收割
来源：生态环境研究中心
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