Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species
文献类型：期刊论文
| 作者 | He, Jianzhong; Li, Tinggang |
| 刊名 | BIORESOURCE TECHNOLOGY
 |
| 出版日期 | 2016-11 |
| 卷号 | 219页码:430-438 |
| 关键词 | Clostridium Butanol Xylan No acetone/ethaNol Formation Carbon Catabolite Derepression |
| DOI | 10.1016/j.biortech.2016.07.138 |
| 英文摘要 | Production of lignocellulosic butanol has drawn increasing attention. However, currently few microorganisms can produce biofuels, particularly butanol, from lignocellulosic biomass via simultaneous saccharification and fermentation. Here we report discovery of a wild-type, mesophilic Clostridium sp. strain MF28 that ferments xylan to produce butanol (up to 3.2 g/L) without the addition of saccharolytic enzymes and without any chemical pretreatments. Application of selective pressure from 2-deoxy-Dglucose facilitated isolation of strain MF28, which exhibits inactivation of genes (gid and ccp genes) responsible for carbon catabolite repression, thus allowing strain MF28 to simultaneously ferment a combination of glucose (30 g/L), xylose (15 g/L), and arabinose (15 g/L) to produce 11.9 g/L of butanol. Strain MF28 possesses several unique features: (i) non-sporulating, (ii) no acetone/ethanol, (iii) complete hemicellulose-binding enzymatic domain, and (iv) absence of carbon catabolite repression. These unique characteristics demonstrate the industrial potential of strain MF28 for cost-effective biofuel generation from lignocellulosic biomass. (C) 2016 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/36262]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | He, Jianzhong,Li, Tinggang. Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species[J]. BIORESOURCE TECHNOLOGY,2016,219:430-438. |
| APA | He, Jianzhong,&Li, Tinggang.(2016).Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species.BIORESOURCE TECHNOLOGY,219,430-438. |
| MLA | He, Jianzhong,et al."Simultaneous saccharification and fermentation of hemicellulose to butanol by a non-sporulating Clostridium species".BIORESOURCE TECHNOLOGY 219(2016):430-438. |
入库方式： OAI收割
来源：生态环境研究中心
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