Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME
文献类型：期刊论文
| 作者 | Du, Huili; Yin, Naiyi; Sun, Guoxin; Cui, Yanshan; Zhang, Zhennan; Cai, Xiaolin; Li, Zejiao |
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2016-10-01 |
| 卷号 | 566页码:1670-1677 |
| 关键词 | Arsenic Bioaccessibility Gut Microbiota Speciation In Vitro Soil |
| DOI | 10.1016/j.scitotenv.2016.06.071 |
| 英文摘要 | Arsenic (As) speciation analysis is essential when evaluating the risks upon oral exposure to As-contaminated soils. In this study, we first investigated the variability in the As bioaccessibility and speciation using a combination of five common in vitro methods (SBRC, PBET, DIN, UBM and IVG) (gastric and small intestinal phases) and the SHIME model (colon phase). Our results indicate that the As bioaccessibility varies in the colon phase. An increase in the As bioaccessibility for SBRC and PBET, and a decrease for UBM and IVG were observed in the colon phase. In addition, we found different extents of methylation and large amounts of arsenite [As(III)] due to microbial reduction in the colon digests. The UBM-SHIME method displayed a higher methylation percentage of 13.5-82.1%, but a lower methylation percentage of 0.2-21.8% was observed in the SBRC-SHIME method. Besides, The MMA(V) levels in the colon digests were positively correlated with those of As( III) and DMA(V), so DMA(V) can be considered an indicator to evaluate the As metabolic speed of in vitro cultured human gut microbiota. Based on the standard reference soil of NIST 2710a, the As bioaccessibility in the colon phase of PBET-SHIME and SBRC-SHIME were the closest to the in vivo results. Combining in vitro methods and SHIME will remarkably affect the accurate assessment of potential risks to human health associated with oral exposure to soil As. (C) 2016 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/36266]  |
| 专题 | 生态环境研究中心_土壤环境科学实验室 |
| 推荐引用方式 GB/T 7714 | Du, Huili,Yin, Naiyi,Sun, Guoxin,et al. Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2016,566:1670-1677. |
| APA | Du, Huili.,Yin, Naiyi.,Sun, Guoxin.,Cui, Yanshan.,Zhang, Zhennan.,...&Li, Zejiao.(2016).Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME.SCIENCE OF THE TOTAL ENVIRONMENT,566,1670-1677. |
| MLA | Du, Huili,et al."Variability of arsenic bioaccessibility and metabolism in soils by human gut microbiota using different in vitro methods combined with SHIME".SCIENCE OF THE TOTAL ENVIRONMENT 566(2016):1670-1677. |
入库方式： OAI收割
来源：生态环境研究中心
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