Functional Characterization of a Novel Amidase Involved in Biotransformation of Triclocarban and its Dehalogenated Congeners in Ochrobactrum sp TCC-2
文献类型：期刊论文
| 作者 | Qiu, Jiguo; Wang, Aijie; Liang, Bin; Yun, Hui; Zhang, Long; Zhao, Youkang; Jiang, Jiandong |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2017-01-03 |
| 卷号 | 51期号:1页码:291-300 |
| 英文摘要 | Haloaromatic antimicrobial triclocarban (3,4,4'-trichlorocarbanilide, TCC) is a refractory contaminant which is frequently deteded in various aquatic and sediment environments globally. However, few TCC-degrading communities or pure cultures have been documented, and functional enzymes involved in TCC biodegradation hitherto have-not yet been characterized. In this study, a bacterial strain, Ochrobactrum sp. TCC-2, capable of degrading TCC under both aerobic and anaerobic conditions was isolated from a sediment sample. A novel amidase gene (tccA); responsible for the hydrolysis of the two amide bonds of TCC and its dehalogenated congeners 4,4'-dichlorocarbanilide (DCC) and carbanilide (NCC) to more biodegradable chloroaniline or aniline products; was cloned and characterized. TccA shares low amino acid sequence identity (27 to 38%) with other biochemically characterized amidases and contains the conserved catalytic triad (Ser-Ser-Lys) of the arnidase signature enzyme family. TccA was stable over a pH range of 5.0 to 10.0 and at temperatures lower than 60 degrees C, and it was constitutively expressed in strain TCC-2. In contrast to the halogenated TCC and DCC, the nonchlorinated NCC was the preferred substrate for TccA. TccA also had hydrolysis activity to a :broad spectrum of amide bonds in herbicides, insecticides, and chemical intermediates. The constitutive expression and broad substrate spectrum of TccA suggested strain TCC-2 may be potentially useful for bioremediation applications. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39633]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Qiu, Jiguo,Wang, Aijie,Liang, Bin,et al. Functional Characterization of a Novel Amidase Involved in Biotransformation of Triclocarban and its Dehalogenated Congeners in Ochrobactrum sp TCC-2[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2017,51(1):291-300. |
| APA | Qiu, Jiguo.,Wang, Aijie.,Liang, Bin.,Yun, Hui.,Zhang, Long.,...&Jiang, Jiandong.(2017).Functional Characterization of a Novel Amidase Involved in Biotransformation of Triclocarban and its Dehalogenated Congeners in Ochrobactrum sp TCC-2.ENVIRONMENTAL SCIENCE & TECHNOLOGY,51(1),291-300. |
| MLA | Qiu, Jiguo,et al."Functional Characterization of a Novel Amidase Involved in Biotransformation of Triclocarban and its Dehalogenated Congeners in Ochrobactrum sp TCC-2".ENVIRONMENTAL SCIENCE & TECHNOLOGY 51.1(2017):291-300. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。