Microbe-microbe interactions trigger Mn(II)-oxidizing gene expression
文献类型：期刊论文
| 作者 | Liang, Jinsong; Bai, Yaohui; Men, Yujie; Qu, Jiuhui |
| 刊名 | ISME JOURNAL
 |
| 出版日期 | 2017 |
| 卷号 | 11期号:1页码:67-77 |
| 英文摘要 | Manganese (Mn) is an important metal in geochemical cycles. Some microorganisms can oxidize Mn (II) to Mn oxides, which can, in turn, affect the global cycles of other elements by strong sorption and oxidation effects. Microbe-microbe interactions have important roles in a number of biological processes. However, how microbial interactions affect Mn(II) oxidation still remains unknown. Here, we investigated the interactions between two bacteria (Arthrobacter sp. and Sphingopyxis sp.) in a co-culture, which exhibited Mn(II)-oxidizing activity, although neither were able to oxidize Mn(II) in isolation. We demonstrated that the Mn(II)-oxidizing activity in co-culture was most likely induced via contact-dependent interactions. The expressed Mn(II)-oxidizing protein in the co-culture was purified and identified as a bilirubin oxidase belonging to strain Arthrobacter. Full sequencing of the bilirubin oxidase-encoding gene (boxA) was performed. The Mn(II)-oxidizing protein and the transcripts of boxA were detected in the co-culture, but not in either of the isolated cultures. This indicate that boxA was silent in Arthrobacter monoculture, and was activated in response to presence of Sphingopyxis in the co-culture. Further, transcriptomic analysis by RNA-Seq, extracellular superoxide detection and cell density quantification by flow cytometry indicate induction of boxA gene expression in Arthrobacter was co-incident with a stress response triggered by co-cultivation with Sphingopyxis. Our findings suggest the potential roles of microbial physiological responses to stress induced by other microbes in Mn(II) oxidation and extracellular superoxide production. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/39716]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Liang, Jinsong,Bai, Yaohui,Men, Yujie,et al. Microbe-microbe interactions trigger Mn(II)-oxidizing gene expression[J]. ISME JOURNAL,2017,11(1):67-77. |
| APA | Liang, Jinsong,Bai, Yaohui,Men, Yujie,&Qu, Jiuhui.(2017).Microbe-microbe interactions trigger Mn(II)-oxidizing gene expression.ISME JOURNAL,11(1),67-77. |
| MLA | Liang, Jinsong,et al."Microbe-microbe interactions trigger Mn(II)-oxidizing gene expression".ISME JOURNAL 11.1(2017):67-77. |
入库方式： OAI收割
来源：生态环境研究中心
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