Integrated Microfluidic Chip for Rapid Capture and Drug Screening of Cancer Cells from Peripheral Blood
文献类型：期刊论文
| 作者 | Zongbin Liu; Weiliang Shu; Rui Chen; Hongtao Feng; Zongqian Hu; Geng Tian; Yuanxiang Wang; Yan Chen; Aixue Liu |
| 刊名 | Integrative Cancer Biology & Research
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Objective: Circulating tumor cells (CTCs) play a key role in metastasis. The detection and characterization of CTCs have important implications for diagnosis and prognosis. However, it is challenging to capture the rare CTCs and do the downstream analysis. The objective of this paper is to develop an integrated microfluidic chip to isolate, capture and culture cancer cells from human whole blood for anticancer drug screening. Methods: A microfluidic system with the combination of deterministic lateral displacement array and affnity based capture is used for the isolation and capture of cancer cells. Deterministic lateral displacement is used to isolate cancer cells from whole blood samples utilizing the size differences between cancer cells and blood cells. The isolated cancer cells are further captured by EpCAM modifed surface. Results: The whole process, including cancer cell capture, culture and drug screening, is performed within an integrated microfluidic chip with simple operation. A capture yield of 90% was achieved at an optimized flow rate of 12 mL/h. The captured cancer cells were capable of proliferation. After cultivation, cancer cells were analyzed for anticancer drug screening with the anti-cancer drugs, MMC and TAM. Results indicated that MMC had a greater cytotoxic effect on cancer cells than TAM. Conclusions: We have demonstrated the feasibility of CTC capture, cultivation and anti-cancer drug screening complied sequentially into a single device. The system has the combined advantages of fast CTC isolation and effcient cancer cell capture, and maintained cell viability for downstream analysis. The microfluidic device with DLD structure and antibody modifed architecture may provide an attractive platform for the use of personalized medicine for cancer patients |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14280]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 推荐引用方式 GB/T 7714 | Zongbin Liu,Weiliang Shu,Rui Chen,et al. Integrated Microfluidic Chip for Rapid Capture and Drug Screening of Cancer Cells from Peripheral Blood[J]. Integrative Cancer Biology & Research,2018. |
| APA | Zongbin Liu.,Weiliang Shu.,Rui Chen.,Hongtao Feng.,Zongqian Hu.,...&Aixue Liu.(2018).Integrated Microfluidic Chip for Rapid Capture and Drug Screening of Cancer Cells from Peripheral Blood.Integrative Cancer Biology & Research. |
| MLA | Zongbin Liu,et al."Integrated Microfluidic Chip for Rapid Capture and Drug Screening of Cancer Cells from Peripheral Blood".Integrative Cancer Biology & Research (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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