Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL
文献类型：期刊论文
| 作者 | Zheng, Hairong; Li, Yuezhou; Duan, Shumin; Ye, Jia; Tang, Siyang; Meng, Long; Li, Xia; Wen, Xiaoxu; Chen, Sihan; Niu, Lili |
| 刊名 | NANO LETTERS
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Externally controlling the excitation of a neuronal subset through ion channels activation can modulate the firing pattern of an entire neural circuit in vivo. As nanovalves in the cell membrane, ion channels can be opened by light (optogenetics) or ultrasonic (sonogenetics) means. A thoroughly analyzed force sensor is the Escherichia coli mechanosensitive channel of large conductance (MscL). Here we expressed MscL in rat hippocampal neurons in a primary culture and showed that it could be activated by low-pressure ultrasound pulses. The gain-of-function mutation, I92L, sensitized MscL's sonic response, triggering action potentials at a peak negative pressure as low as 0.25 MPa. Further, the I92L MscL reliably elicited individual spikes by timed brief pulses, making excitation programmable. Because MscL opens to tension in the lipid bilayer, requiring no other proteins or ligands, it could be developed into a general noninvasive sonogenetic tool to manipulate the activities of neurons or other cells and potential nanodevices. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14340]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 推荐引用方式 GB/T 7714 | Zheng, Hairong,Li, Yuezhou,Duan, Shumin,et al. Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL[J]. NANO LETTERS,2018. |
| APA | Zheng, Hairong.,Li, Yuezhou.,Duan, Shumin.,Ye, Jia.,Tang, Siyang.,...&Shu, Qiang.(2018).Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL.NANO LETTERS. |
| MLA | Zheng, Hairong,et al."Ultrasonic Control of Neural Activity through Activation of the Mechanosensitive Channel MscL".NANO LETTERS (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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