Biomechanical Heterogeneity of Living Cells: Comparison between 2 Atomic Force Microscopy and Finite Element Simulation
文献类型：期刊论文
| 作者 | Florian Stadler; Massimiliano Galluzzi; Bokai Zhang; Yu-Lin Shen; Guanlin Tang |
| 刊名 | Langmuir
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Atomic force microscopy (AFM) indentation is a popular method for characterizing the micromechanical properties of soft materials such as living cells. However, the mechanical data obtained from deep indentation measurements can be difficult and problematic to interpret as a result of the complex geometry of a cell, the nonlinearity of indentation contact, and constitutive relations of heterogeneous hyperelastic soft components. Living MDA-MB-231 cells were indented by spherical probes to obtain morphological and mechanical data that were adopted to build an accurate finite element model (FEM) for a parametric study. Initially, a 2D- axisymmetric numerical model was constructed with the main purpose of understanding the effect of geometrical and mechanical properties of constitutive parts such as the cell body, nucleus, and lamellipodium. A series of FEM deformation fields were directly compared with atomic force spectroscopy in order to resolve the mechanical convolution of heterogeneous parts and quantify Young’s modulus and the geometry of nuclei. Furthermore, a 3D finite element model was constructed to investigate indentation events located far from the axisymmetric geometry. In this framework, the joint FEM/AFM approach has provided a useful methodology and a comprehensive characterization of the heterogeneous structure of living cells, emphasizing the deconvolution of geometrical structure and the true elastic modulus of the cell nucleus. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14406]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 推荐引用方式 GB/T 7714 | Florian Stadler,Massimiliano Galluzzi,Bokai Zhang,et al. Biomechanical Heterogeneity of Living Cells: Comparison between 2 Atomic Force Microscopy and Finite Element Simulation[J]. Langmuir,2018. |
| APA | Florian Stadler,Massimiliano Galluzzi,Bokai Zhang,Yu-Lin Shen,&Guanlin Tang.(2018).Biomechanical Heterogeneity of Living Cells: Comparison between 2 Atomic Force Microscopy and Finite Element Simulation.Langmuir. |
| MLA | Florian Stadler,et al."Biomechanical Heterogeneity of Living Cells: Comparison between 2 Atomic Force Microscopy and Finite Element Simulation".Langmuir (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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