Analysis of differential uptake of iron oxide nanoparticles by macrophages via magnetophoretic sorting in a trapezoidal microchannel
文献类型：会议论文
| 作者 | Shen FS(申凤善); Je-Kyun Park |
| 出版日期 | 2018 |
| 会议日期 | 2018 |
| 会议地点 | 台湾 |
| 英文摘要 | Magnetic iron oxide nanoparticles have a wide range of properties which make them useful in biological applications such as magnetic resonance imaging (MRI) contrast agents, separation of magnetically labeled cells, and magnetic heat generators for hyperthermia. To exploit these applications, the cells must be labeled with sufficient amounts of magnetic nanoparticles via endocytosis. Among the different cell types, noninvasive imaging of macrophage activity has raised increasing interest in the diagnosis of disease involving inflammation, infection, and tissue degeneration. Macrophages reside in virtually all tissues, and the tissue environment will orient macrophages to polarized different status. In vivo, it has been shown that macrophages have at least two opposite polarization states with distinct functions. Classically activated or M1 macrophages have a pro-inflammatory action and can be activated by IFNγ together with bacterial lipopolysaccharides (LPS). In contrast, alternatively activated or M2 macrophages promoted by Il-4 and IL-13 generally thought to exert anti-inflammatory functions and to promote wound healing and angiogenesis. Recent findings showed that variations in macrophage activation status resulted in significant differences in uptake of nanoparticles, with high uptake in so-called M1 macrophages and low uptake in M2 cells. Most studies focused on investigating the impact of particle size, functional groups and the presence of serum on differential uptake of nanoparticles in two macrophage subtypes (M1 and M2) [1]. However, cells classified as the identical subtype also exhibit significant heterogeneity in nanoparticle uptake due to the cell-to-cell variation in uptake capabilities. Therefore, it is also crucial to have an in-depth focus on the extent of heterogeneous uptake of within subtypes. Previously, we reported efficient magnetic sorting based on cellular magnetic loading using a trapezoidal microchannel [2]. The bulk cell population separated into several subpopulations according to their iron oxide loading ranging from cells being heavily loaded to the subpopulation with miniscule amount of iron. Exploiting the magentophoretic device mentioned before, herein we proposed the assessment of the uptake variation of subpopulations according to their iron content. The whole producers illustrated in Figure 1. To understand the differential uptake by macrophage polarization, the THP-1 cells were differentiated to M0 with PMA (phorbol 12-myristate 13-acetate), and then M0 cells activated with corresponding treatment and polarized into M1 and M2 status. Then the three subtypes of M0, M1 and M2 cells were labelled with iron oxide nanoparticles via internalization processes. The three subtypes of cells which show significant heterogeneity in intracellular iron content were independently separated into several subpopulations based on their magnetic loading. The sorted subpopulations enable various post analysis as a function of the uptake capacities. The Figure 2 shows the characterization of THP-1 differentiation and polarization. The qualitative (Figure 3) and quantitative (Figure 4) assessment of iron content of seven sorted subpopulations were carried out using M0 cells. The extent of heterogeneous uptake within subtypes is determined by measuring the ratio of cells from each subpopulation outlet (Figure 5), providing more detailed information. |
| URL标识 | 查看原文 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14531]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 推荐引用方式 GB/T 7714 | Shen FS,Je-Kyun Park. Analysis of differential uptake of iron oxide nanoparticles by macrophages via magnetophoretic sorting in a trapezoidal microchannel[C]. 见:. 台湾. 2018. |
入库方式： OAI收割
来源：深圳先进技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。