A novel 3D printing composite scaffold with phytoestrogenic osteopomotive puerarin for potential bone regeneration
文献类型：会议论文
| 作者 | Mauro Alini; Xinluan Wang; Ling Li; Huijuan Cao; Cuishan Huang; Jiani Wang; Long Li; Yuxiao Lai; Sibylle Grad; Ling Qin |
| 出版日期 | 2018 |
| 会议日期 | 2018 |
| 会议地点 | Kyoto |
| 英文摘要 | Background: Phytoestrogen puerarin was found to be able to promote osteogenesis. In this study, 0.5% (by weight) of puerarin was incorporated into the PLGA/TCP scaffold (PT) by 3D priting to form a composite PTP for promoting osteogenesis. Method: The porosity of the composite scaffolds was tested by micro CT. High performance liquid chromatography was used to detect the puerarin in vitro release from PTP. MC 3T3-E1 Cells were used to test the osteogenic effect of the scaffold. Results: The porosity of PTP is 74.9%, and it has different pores (400~500 µm for macro pores or 5-50 µm for micro pores). Puerarin was rapidly released at the first 10 days and then kept steadily during 10 to 59 days, after that it released a little faster than before. Furthermore, PTP scaffolds appeared a better biocompatibility than PT, and its degradation medium could promote maturation of mineralization of MC3T3-E1 through regulating osteoblastic differentiation markers (Runx2, Osterix, OPN, OC and BSP) mRNA expression and stimulate ALP activity. Conclusion: Accordingly, puerarin was able to incorporated into the degradable PLGA/TCP scaffolds, slowly released from the scaffolds during degradation and exerted osteogenic effects in vitro. The in vivo study is ongoing. As a long-time clinical used drug, puerarin-incorporated scaffold has highly translational potential for the future application in bone regeneration. |
| URL标识 | 查看原文 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14584]  |
| 专题 | 深圳先进技术研究院_医工所 |
| 推荐引用方式 GB/T 7714 | Mauro Alini,Xinluan Wang,Ling Li,et al. A novel 3D printing composite scaffold with phytoestrogenic osteopomotive puerarin for potential bone regeneration[C]. 见:. Kyoto. 2018. |
入库方式： OAI收割
来源：深圳先进技术研究院
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