Dissecting complicated viral spreading of enterovirus 71 using in situ bioorthogonal fluorescent labeling
文献类型：期刊论文
| 作者 | Hong Pan; Xiangjie Yao; Weihua Chen; Fangfang Wang; Huamei He; Lanlan Liu; Yaqing He; Jinquan; Puzi Jiang; Renli Zhang |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Enterovirus 71 (EV71), the major pathogen of hand-foot-and-mouth disease (HFDM), can cause severe neurological and respiratory manifestations in young children. Viral spread route and tissue tropism are key factors contributing to different pathogenicity of EV71, however it remains a challenge to dynamically visualize EV71 infection in vivo. The present study applies an in situ bioorthogonal fluorescent labeling strategy to track clinically isolated EV71 strains with different pathogenicity in neonatal mice. The results show that the in situ labeling strategy effectively captures EV71 viruses through in vivo bioorthogonal reaction in multiple infected organs without interfering viral spread and tissue tropism. More importantly, the in situ labeling reveals different viral dynamics, dissemination, and tissue tropism of severe case EV71 (SC-EV71) and mild case EV71 (MC-EV71), consistent with their different pathogenicity in HFDM patients. Compared with MC-EV71, SC-EV71 not only enters the blood circulation and spreads out more quickly, but also shows more significant neuronal and respiratory tropism, which certainly contribute severe neurological complications and clinical manifestations in the patient. Hence, the in situ bioorthogonal fluorescent labeling is a plausible strategy to dissect complicated process of EV71 viral spread in the early stage of infection, thereby offering great opportunities to understand its pathogenesis and develop anti-viral drugs |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14610]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 推荐引用方式 GB/T 7714 | Hong Pan,Xiangjie Yao,Weihua Chen,et al. Dissecting complicated viral spreading of enterovirus 71 using in situ bioorthogonal fluorescent labeling[J]. BIOMATERIALS,2018. |
| APA | Hong Pan.,Xiangjie Yao.,Weihua Chen.,Fangfang Wang.,Huamei He.,...&Lintao Cai.(2018).Dissecting complicated viral spreading of enterovirus 71 using in situ bioorthogonal fluorescent labeling.BIOMATERIALS. |
| MLA | Hong Pan,et al."Dissecting complicated viral spreading of enterovirus 71 using in situ bioorthogonal fluorescent labeling".BIOMATERIALS (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。