Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots
文献类型：期刊论文
| 作者 | Chen, Chi; Ao, Lijiao; Wu, Yu-Tang; Cifliku, Vjona; Dos Santos, Marcelina Cardoso; Bourrier, Emmanuel; Delbianco, Martina; Parker, David; Zwier, Jurriaan M.; Huang, Liang |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Fluorescence barcoding based on nanoparticles provides many advantages for multiparameter imaging. However, creating different concentration-independent codes without mixing various nanoparticles and by using single-wavelength excitation and emission for multiplexed cellular imaging is extremely challenging. Herein, we report the development of quantum dots (QDs) with two different SiO2 shell thicknesses (6 and 12nm) that are coated with two different lanthanide complexes (Tb and Eu). FRET from the Tb or Eu donors to the QD acceptors resulted in four distinct photoluminescence (PL) decays, which were encoded by simple time-gated (TG) PL intensity detection in three individual temporal detection windows. The well-defined single-nanoparticle codes were used for live cell imaging and a one-measurement distinction of four different cells in a single field of view. This single-color barcoding strategy opens new opportunities for multiplexed labeling and tracking of cells. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14614]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 推荐引用方式 GB/T 7714 | Chen, Chi,Ao, Lijiao,Wu, Yu-Tang,et al. Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018. |
| APA | Chen, Chi.,Ao, Lijiao.,Wu, Yu-Tang.,Cifliku, Vjona.,Dos Santos, Marcelina Cardoso.,...&Hildebrandt, Niko.(2018).Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. |
| MLA | Chen, Chi,et al."Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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