Novel cyanine 5 conjugated magnetic beads for rapidly and efficiently detection of OATP1B3 over-expressed circulating tumor cells
文献类型：期刊论文
| 作者 | Yayun Wu; Lihua Zhou; Xiaoqing Meng; Zhihong Sun; Xinghua Xi; Guolin Li; Ping Gong; Lintao Cai |
| 刊名 | Nanomedicine: Nanotechnology, Biology, and Medicine
 |
| 出版日期 | 2018 |
| 文献子类 | 会议论文 |
| 英文摘要 | Circulating tumor cells (CTCs) are cancer cells in peripheral blood spontaneously released by solid tumor or metastatic lesions, sometimes caused by surgery operation. Recently, CTCs detection technologies including PCR, fluorescenceactivated cell scanning/sorting (FACS), magnetic-activated cell separation (MACS) and Cell Search have been widely used in research and clinic. Specially, Cell Search is the only clinically applied CTCs capture technology via EPCAM antibody modified magnetic beads. However, some defects limited its Figure 1. (A) SEM image, (B) XRD spectrum, (C) Cell viability of NaTb(SO4)2•H2O nanospheres and (D, H) TEM images of ultrathin sections of HeLa cells incubated with NaTb(SO4)2•H2O for 10 h. (E) and (F) demonstrate fluorescence quench of NaTb(SO4)2•H2O nanospheres dispersed in Fe3+ or Fe2+ ions solutions (λEX=221 nm). (G) shows fluorescent spectra (λEX=221 nm) of HeLa cells co-cultured with NaTb(SO4)2•H2O nanospheres with or without coexistence of Fe3+ ions after digested by trypsin, rinsed by and re-dispersed in PBS. ChinaNanomedicine 2016 / Nanomedicine: Nanotechnology, Biology, and Medicine 14 (2018) 1743–1885 1789 clinical application including unstable activity of antibody and changeable biomarkers of metastatic cancer cells. Some special cyanine dyes have been reported to display excellent targeting property by transporting of OATPs which over-expressed in most tumor cells. In this work, we firstly discovered superior targeting property of Cyanine 5, a commercial fluorescent cyanine dye. Accordingly, we developed a new fluorescent diagnostic technique that combines Cyanine 5 with magnetic beads for dual separation of OATP1B3 over-expressed CTCs. This technique overcame defects of antibody and showed great potential for further application of clinical capture of CTCs |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14623]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 推荐引用方式 GB/T 7714 | Yayun Wu,Lihua Zhou,Xiaoqing Meng,et al. Novel cyanine 5 conjugated magnetic beads for rapidly and efficiently detection of OATP1B3 over-expressed circulating tumor cells[J]. Nanomedicine: Nanotechnology, Biology, and Medicine,2018. |
| APA | Yayun Wu.,Lihua Zhou.,Xiaoqing Meng.,Zhihong Sun.,Xinghua Xi.,...&Lintao Cai.(2018).Novel cyanine 5 conjugated magnetic beads for rapidly and efficiently detection of OATP1B3 over-expressed circulating tumor cells.Nanomedicine: Nanotechnology, Biology, and Medicine. |
| MLA | Yayun Wu,et al."Novel cyanine 5 conjugated magnetic beads for rapidly and efficiently detection of OATP1B3 over-expressed circulating tumor cells".Nanomedicine: Nanotechnology, Biology, and Medicine (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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