siRNA-mediated c-Rel knockdown ameliorates collagen-induced arthritis in mice
文献类型：期刊论文
| 作者 | Qingguo Ruan; Tingting Fan; Fuhua Zhong; Ruiling Liu; YOUHAI H Chen; Ting Wang |
| 刊名 | Journal of Immunology
 |
| 出版日期 | 2018 |
| 文献子类 | 会议论文 |
| 英文摘要 | Previous studies have shown that inflammatory mediators involved in the development of rheumatoid arthritis (RA) are regulated by the Rel/nuclear factor-κB (Rel/NF-κB) transcription factor family. c-Rel, a member of the Rel/NF-κB family that is preferentially expressed by immune cells, is a risk factor for several inflammatory diseases including RA. In the current study, we investigated whether targeting c-Rel can be used to treat collagen-induced arthritis, an animal model for RA. c-Rel specific siRNA (siRel) delivered by nanoparticles was used to knockdown the expression of c-Rel. Our results showed that siRel treatment significantly ameliorated collagen-induced arthritis. Further study revealed that c-Rel expression in the dendritic cells and macrophages from mice treated with siRel was significantly down-regulated. Consistent with the phenotypical result, the expression of inflammatory cytokines TNF-α, IL-1β, IL-6, IL-12 and IL-23 by peritoneal macrophages and splenocytes were significantly decreased. In addition, attenuated systemic and collagen-specific Th1 and Th17 immune responses were observed. Furthermore, we found that the expression of inflammatory cytokines was significantly down-regulated and the infiltration of CD3+ T cells and F4/80+ macrophages was markedly reduced in hind paws of mice treated with siRel. Collectively, our study provides strong evidence that siRNA-mediated c-Rel knockdown can suppress the development of collagen-induced arthritis in mice. Therefore, blocking c-Rel may represent an attracting strategy for the treatment of human rheumatoid arthritis. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14636]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 推荐引用方式 GB/T 7714 | Qingguo Ruan,Tingting Fan,Fuhua Zhong,et al. siRNA-mediated c-Rel knockdown ameliorates collagen-induced arthritis in mice[J]. Journal of Immunology,2018. |
| APA | Qingguo Ruan,Tingting Fan,Fuhua Zhong,Ruiling Liu,YOUHAI H Chen,&Ting Wang.(2018).siRNA-mediated c-Rel knockdown ameliorates collagen-induced arthritis in mice.Journal of Immunology. |
| MLA | Qingguo Ruan,et al."siRNA-mediated c-Rel knockdown ameliorates collagen-induced arthritis in mice".Journal of Immunology (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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