Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice
文献类型：期刊论文
| 作者 | Xin Li; Guizhong Zhang; Lvyan Liu; Junxin Li; Qian Chen; Shiran Huang; Yutao Li; Xiaochun Wan |
| 刊名 | Biochemical and Biophysical Research Communications
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| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Background: Conventional hepatitis B virus (HBV) vaccines fail to induce protective antibody titers in 5-10% of immune-competent vaccines. Therefore, safe and effective HBV vaccines are still clinically needed. Methods: In this study, we developed a plasmid DNA vaccine encoding CD317 single-chain fragment variable (α317scFv) linked with the hepatitis B surface antigen (HBsAg) and detected the humoral and cellular immune responses elicited by this vaccine in BALB/c mice. Results: Vaccination with this fusion DNA vaccine in BALB/c mice induced more robust antiviral T cell and antibody immunity against HBsAg. Compared with mice vaccinated with control vaccine encoding HBsAg, the level of serum-circulating anti-HBsAg antibody (HBsAb) was nearly double in fusion DNA-vaccinated mice. More interesting, splenic lymphocytes isolated from fusion DNA-vaccinated mice showed more potent proliferation and IFN-γ production after being re-stimulated with recombinant HBsAg in vitro. Conclusion: Taken together, our results reveal that the fusion DNA vaccine can induce more effective immunological protection against HBV, and is a promising candidate for preventing HBV infection. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14644]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 推荐引用方式 GB/T 7714 | Xin Li,Guizhong Zhang,Lvyan Liu,et al. Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice[J]. Biochemical and Biophysical Research Communications,2018. |
| APA | Xin Li.,Guizhong Zhang.,Lvyan Liu.,Junxin Li.,Qian Chen.,...&Xiaochun Wan.(2018).Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice.Biochemical and Biophysical Research Communications. |
| MLA | Xin Li,et al."Vaccination with a DNA vaccine encoding CD317-targeting HBs antigen elicits enhanced immunity in mice".Biochemical and Biophysical Research Communications (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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