Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing
文献类型：期刊论文
| 作者 | Zhou, Wenhua; Cui, Haodong; Ying, Liming; Yu, Xue-Feng |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | A biodegradable two-dimensional (2D) delivery platform based on loading black phosphorus nanosheets (BPs) with Cas9 ribonucleoprotein engineered with three nuclear localization signals (NLSs) at C terminus (Cas9N3) is successfully established. The Cas9N3-BPs enter cells effectively via membrane penetration and endocytosis pathways, followed by a BPs biodegradation-associated endosomal escape and cytosolic releases of the loaded Cas9N3 complexes. The Cas9N3-BPs thus provide efficient genome editing and gene silencing invitro and invivo at a relatively low dose as compared with other nanoparticle-based delivery platforms. This biodegradable 2D delivery platform offers a versatile cytosolic delivery approach for CRISPR/Cas9 ribonucleoprotein and other bioactive macromolecules for biomedical applications. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14661]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 推荐引用方式 GB/T 7714 | Zhou, Wenhua,Cui, Haodong,Ying, Liming,et al. Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2018. |
| APA | Zhou, Wenhua,Cui, Haodong,Ying, Liming,&Yu, Xue-Feng.(2018).Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. |
| MLA | Zhou, Wenhua,et al."Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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