VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors.
文献类型：期刊论文
| 作者 | Zhong-Lin Xiao; Songjun Li; Yan Yu; Mengxia Li; Jie Chen; Feng Wang; Jian Zhang; Weifen Deng; Qing Yang; Xiujun Fan |
| 刊名 | Molecular and Cellular Biochemistry
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Studies have shown that sFlt-1 overproduction stimulated by excess VEGF of deciduous origin in trophoblasts can cause preeclampsia. However, the mechanism underlying how VEGF regulates sFtl-1 expression in trophoblasts remains unknown. To address this issue, JEG3 and HTR-8/SV neo (HTR8) trophoblast cell lines were used to investigate the signaling pathways involved in the regulation of sFlt-1 production via VEGF overexpression in vitro. JEG3 (VEGF–GFP–JEG3, V-J) and HTR8 (VEGF–GFP–HTR8, V-H) cells overexpressing VEGF165 were established by infecting the JEG3 and HTR8 cell lines with lentivirus expressing VEGF165. Both the mRNA and protein levels of VEGF and sFlt-1 were dramatically up-regulated in the V-J and V-H cells compared to the JEG3 and HTR8 cells, and they were significantly decreased after treatment with an Flt-1 receptor inhibitor (MK-2461), a KDR receptor inhibitor (XL-184), or an Flt-1 and KDR receptor inhibitor (ABT-869). The mRNA levels of sFlt-1, Flt-1, and KDR were increased in V-H cells after treatment, and the VEGF-A mRNA levels were also elevated. The migration and invasion abilities of JEG3 and HTR8 cells were decreased after VEGF overexpression, and this reduction could be reversed with VEGF receptor inhibitor treatment. In addition, after the different treatments, the cell migration rates of V-J cells were significantly increased compared with the control treatment. Taken together, these results indicate that sFlt-1 up-regulation by VEGF may be mediated by the VEGF/Flt-1 and/or VEGF/KDR signaling pathways. However, elucidating which pathway plays this key role requires further investigatio |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14690]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 推荐引用方式 GB/T 7714 | Zhong-Lin Xiao,Songjun Li,Yan Yu,et al. VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors.[J]. Molecular and Cellular Biochemistry,2018. |
| APA | Zhong-Lin Xiao.,Songjun Li.,Yan Yu.,Mengxia Li.,Jie Chen.,...&Xiujun Fan.(2018).VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors..Molecular and Cellular Biochemistry. |
| MLA | Zhong-Lin Xiao,et al."VEGF-A regulates sFlt-1 production in trophoblasts through both Flt-1 and KDR receptors.".Molecular and Cellular Biochemistry (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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