Cytotoxicity induced by iodinated haloacetamides via ROS accumulation and apoptosis in HepG-2 cells
文献类型：期刊论文
| 作者 | Yu, Haiying; Liang, Yan; Hong, Huachang; Wu, Huan; Chen, Jiao; Wu, Binbin; Yan, Bin |
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Iodinated haloacetamides (I-HAcAms) are emerging disinfection by-products and have received great concern due to their extremely high health risk. Previous studies have demonstrated the cytotoxicity of I-HAcAms, but the biological mechanism remained unclear. In this study, cytotoxicity mechanisms of 4 I-HAcAms species were preliminarily examined using HepG-2 cells. The results showed that the cytotoxicity could be ranked as follows: diiodoacetamide (DIAcAm)> iodoacetamide (IAcAm)> bromoiodoacetamide (BIAcAm)> chloroiodoacetamide (CIAcAm). Reactive oxygen species (ROS) and apoptosis played an important role in the cytotoxicity for all I-HAcAms species. Moreover, the ROS and cytotoxicity could be completely reversed by the addition of an antioxidant (N-acetylcysteine (NAC)), but the apoptosis could not. Specifically, the apoptosis induced by DIAcAm and IAcAm was partially reversed by NAC, suggesting that in addition to ROS, other pathways were also possible; While For BIAcAm and CIAcAm, the apoptosis was not reversed by NAC at all, which is potentially due to ROS-independent pathways. The apoptosis mechanisms were further analyzed via Bax and Bcl-2 gene expression and the corresponding protein expression in HepG-2 cells, that mitochondrial pathway was important in the apoptosis of HepG-2 cells induced by all I-HAcAms species. Overall, the mitochondrial pathway provided a potential explanation for BIAcAm and CIAcAm-induced apoptosis, while both ROS and mitochondrial pathways explained DIAcAm and IAcAm-induced apoptosis |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14740]  |
| 专题 | 深圳先进技术研究院_医药所 |
| 推荐引用方式 GB/T 7714 | Yu, Haiying,Liang, Yan,Hong, Huachang,et al. Cytotoxicity induced by iodinated haloacetamides via ROS accumulation and apoptosis in HepG-2 cells[J]. ENVIRONMENTAL POLLUTION,2018. |
| APA | Yu, Haiying.,Liang, Yan.,Hong, Huachang.,Wu, Huan.,Chen, Jiao.,...&Yan, Bin.(2018).Cytotoxicity induced by iodinated haloacetamides via ROS accumulation and apoptosis in HepG-2 cells.ENVIRONMENTAL POLLUTION. |
| MLA | Yu, Haiying,et al."Cytotoxicity induced by iodinated haloacetamides via ROS accumulation and apoptosis in HepG-2 cells".ENVIRONMENTAL POLLUTION (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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