YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast
文献类型：期刊论文
| 作者 | Dai, Junbiao; Garcia-Ruiz, Eva; Auxillos, Jamie; Li, Tianyi; Cai, Yizhi |
| 刊名 | ENZYMES IN SYNTHETIC BIOLOGY
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | For many years, researchers have devised elegant techniques to assemble genetic parts into larger constructs. Recently, increasing needs for complex DNA constructs has driven countless attempts to optimize DNA assembly methods for improved efficiency, fidelity, and modularity. These efforts have resulted insimple, robust, standardized, and fast protocols that enable the implementation of high-throughput DNA assembly projects for the fabrication of large synthetic genetic constructs. Recently our groups have developed the YeastFab assembly, a highly efficient method for the design and construction of DNA-building blocks based on the native elements from Saccharomyces cerevisiae. Furthermore, these standardized DNA parts can be readily characterized and assembled into transcriptional units and pathways. In this chapter, we describe the protocols to assemble pathways from characterized standardized yeastparts using YeastFab. |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14881]  |
| 专题 | 深圳先进技术研究院_其他 |
| 推荐引用方式 GB/T 7714 | Dai, Junbiao,Garcia-Ruiz, Eva,Auxillos, Jamie,et al. YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast[J]. ENZYMES IN SYNTHETIC BIOLOGY,2018. |
| APA | Dai, Junbiao,Garcia-Ruiz, Eva,Auxillos, Jamie,Li, Tianyi,&Cai, Yizhi.(2018).YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast.ENZYMES IN SYNTHETIC BIOLOGY. |
| MLA | Dai, Junbiao,et al."YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast".ENZYMES IN SYNTHETIC BIOLOGY (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。