A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes
文献类型：期刊论文
| 作者 | Singh, Netrapal; Yang, Fang; Zhang, Xian-En; Gu, Ning; Huang, Lin; Wang, Dian-Bing |
| 刊名 | NANOSCALE
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Exosomes as nanosized biomarkers hold great potential for the diagnosis of cancer. However, the low concentration of cancer-derived exosomes present in biofluids makes early diagnosis strenuous. Here, we developed a fluorescent biosensing platform, namely a dual signal amplification, for the ultrasensitive detection of leukemia cell-derived exosomes. The protocol consists of three steps: first, leukemia-derived exosomes containing CD63 and nucleolin were captured by anti-CD63 antibody modified magnetic bead conjugates (MB-CD63); then, a DNA primer comprising a nucleolin-recognition aptamer (AS1411) was applied to bind the exosomes which further initiated a rolling circle amplification (RCA) reaction to generate many repeat sequences for hybridization with gold nanoparticle (GNP)-DNA-fluorescent dye (FAM) conjugates (GNP-DNA-FAM); finally, nicking endonuclease (NbBbvCI) assisted target recycling was introduced. As a result, FAM was released from GNP-DNA-FAM conjugates, transformed from the quenching state to the emission state and thus fluorescencesignals continuously accumulated. With this dual signal amplification platform, as low as 1 x 10(2) particles per L exosomes could be detected. Furthermore, we have successfully applied this method for the detection of exosomes in spiked serum samples, indicating a promising tool for clinical application. |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/14886]  |
| 专题 | 深圳先进技术研究院_其他 |
| 推荐引用方式 GB/T 7714 | Singh, Netrapal,Yang, Fang,Zhang, Xian-En,et al. A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes[J]. NANOSCALE,2018. |
| APA | Singh, Netrapal,Yang, Fang,Zhang, Xian-En,Gu, Ning,Huang, Lin,&Wang, Dian-Bing.(2018).A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes.NANOSCALE. |
| MLA | Singh, Netrapal,et al."A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes".NANOSCALE (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。