A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats
文献类型：期刊论文
| 作者 | Gaowei Chen; Kuikui Zhou; Yingjie Zhu; Yongmei Sun |
| 刊名 | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | A major cause of repeated relapses is a craving for the drug. Drug craving increases progressively during the abstinence period, a phenomenon termed incubation of drug craving. Here, we describe a morphine conditioned place preference (CPP) protocol for measuring the incubation of craving in rats. In this protocol, a CPP paradigm mainly employing somatosensory cues is used to establish a long-term reward memory of morphine. A three-chamber CPP box that differs in the texture of the chamber floor is constructed. First, the animals are tested for their baseline preference to the two side chambers for three consecutive days. Then, they are injected intraperitoneally with morphine/saline and put into their non-preferred/preferred chamber for 45 min. After 6 days of conditioning, their preference to the side chambers is tested for 15 min at different time points after the last conditioning session. With this paradigm, the reward memory of morphine could last for at least 18 days. To test whether the above-mentioned protocol can model increased craving, the number of entrances into the two side chambers are counted during the abstinence period. The results show that the entrances increased, suggesting that the CPP paradigm could mimic the incubation of craving. Future studies can employ this model to study neural mechanisms underlying long-term memory and incubation of craving. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/13873]  |
| 专题 | 深圳先进技术研究院_脑所 |
| 推荐引用方式 GB/T 7714 | Gaowei Chen, Kuikui Zhou, Yingjie Zhu,et al. A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats[J]. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,2018. |
| APA | Gaowei Chen, Kuikui Zhou, Yingjie Zhu,&Yongmei Sun.(2018).A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats.JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. |
| MLA | Gaowei Chen,et al."A Conditioned Place Preference Protocol for Measuring Incubation of Craving in Rats".JOVE-JOURNAL OF VISUALIZED EXPERIMENTS (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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