Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
文献类型：期刊论文
| 作者 | Perez-Pi, Irene; Liu, Longying; Shen, Yue; French, Chris; Cai, Yizhi; Auer, Manfred; Dai, Junbiao; Marles-Wright, Jon; Liu, Wei; Luo, Zhouqing |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Exogenous pathway optimization and chassis engineering are two crucial methods for heterologous pathway expression. The two methods are normally carried out step-wise and in a trial-and-error manner. Here we report a recombinase-based combinatorial method (termed "SCRaMbLE-in") to tackle both challenges simultaneously. SCRaMbLE-in includes an in vitro recombinase toolkit to rapidly prototype and diversify gene expression at the pathway level and an in vivo genome reshuffling system to integrate assembled pathways into the synthetic yeast genome while combinatorially causing massive genome rearrangements in the host chassis. A set of loxP mutant pairs was identified to maximize the efficiency of the in vitro diversification. Exemplar pathways of beta-carotene and violacein were successfully assembled, diversified, and integrated using this SCRaMbLE-in method. High-throughput sequencing was performed on selected engineered strains to reveal the resulting genotypeto-phenotype relationships. The SCRaMbLE-in method proves to be a rapid, efficient, and universal method to fast track the cycle of engineering biology. |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/13428]  |
| 专题 | 合成所 |
| 推荐引用方式 GB/T 7714 | Perez-Pi, Irene,Liu, Longying,Shen, Yue,et al. Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods[J]. NATURE COMMUNICATIONS,2018. |
| APA | Perez-Pi, Irene.,Liu, Longying.,Shen, Yue.,French, Chris.,Cai, Yizhi.,...&Tuck, Laura.(2018).Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods.NATURE COMMUNICATIONS. |
| MLA | Perez-Pi, Irene,et al."Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods".NATURE COMMUNICATIONS (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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