Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli
文献类型：期刊论文
| 作者 | Lee, Taek Soon; Liu, Chun-Li; Tian, Tian; Alonso-Gutierrez, Jorge; Garabedian, Brett; Wang, Shuai; Baidoo, Edward E. K.; Benites, Veronica; Chen, Yan; Petzold, Christopher J. |
| 刊名 | BIOTECHNOLOGY FOR BIOFUELS
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | BackgroundAviation fuels are an important target of biofuels research due to their high market demand and competitive price. Isoprenoids have been demonstrated as good feedstocks for advanced renewable jet fuels with high energy density, high heat of combustion, and excellent cold-weather performance. In particular, sesquiterpene compounds (C-15), such as farnesene and bisabolene, have been identified as promising jet fuel candidates.ResultsIn this study, we explored three sesquiterpenesepi-isozizaene, pentalenene and -isocomeneas novel jet fuel precursors. We performed a computational analysis to calculate the energy of combustion of these sesquiterpenes and found that their specific energies are comparable to commercial jet fuel A-1. Through heterologous MVA pathway expression and promoter engineering, we produced 727.9mg/L epi-isozizaene, 780.3mg/L pentalenene and 77.5mg/L -isocomene in Escherichia coli and 344mg/L pentalenene in Saccharomyces cerevisiae. We also introduced a dynamic autoinduction system using previously identified FPP-responsive promoters for inducer-free production and managed to achieve comparable amounts of each compound.ConclusionWe produced tricyclic sesquiterpenes epi-isozizaene, pentalenene and -isocomene, promising jet fuel feedstocks at high production titers, providing novel, sustainable alternatives to petroleum-based jet fuels. |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/13436]  |
| 专题 | 合成所 |
| 推荐引用方式 GB/T 7714 | Lee, Taek Soon,Liu, Chun-Li,Tian, Tian,et al. Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli[J]. BIOTECHNOLOGY FOR BIOFUELS,2018. |
| APA | Lee, Taek Soon.,Liu, Chun-Li.,Tian, Tian.,Alonso-Gutierrez, Jorge.,Garabedian, Brett.,...&Tan, Tianwei.(2018).Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli.BIOTECHNOLOGY FOR BIOFUELS. |
| MLA | Lee, Taek Soon,et al."Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli".BIOTECHNOLOGY FOR BIOFUELS (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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