A novel galactose-PEG-conjugated biodegradable copolymer is an efficient gene delivery vector for immunotherapy of hepatocellular carcinoma
文献类型：期刊论文
| 作者 | Liu, Yi; Liu, Ling; Zong, Zhi-Min; Liu, Qian; Jiang, Shuang-Shuang; Zhang, Qian; Cen, Lan-Qi; Gao, Jian; Gao, Xiao-Ge; Huang, Jian-Dong |
| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Successful immunogene therapy depends not only on the therapeutic gene but also on the gene delivery vector. In this study, we synthesized a novel copolymer consisting of low-molecular-weight polyethylenimine (PEI) cross-linked by myo-inositol (INO) and conjugated with a galactose-grafted PEG chain, named LA-PegPI. We characterized the chemical structure and molecular weight of the copolymer and particle properties of LA-PegPI/pDNA. Furthermore, we showed that LA-PegPl/pDNA polyplexes possessed excellent stability in physiological salt solution, low cytotoxicity,.and high transfection efficiency in the asialoglycoprotein receptor (ASGPR)-positive liver cells in vitro. Importantly, we also showed that through intraperitoneal injection of LA-PegPI/pDNA nanoparticles, the reporter gene was forcefully expressed in the liver hepatocytes of mice. Finally, we documented that intraperitoneal injection of LA-PegPI/pIL15 nanoparticles effectively suppressed tumor growth and prolonged survival time of tumor-bearing mice via activation of CD8(+) T cells and NK cells and upregulation of the cytokines IFN-gamma, TNF, and IL12 in an orthotopic hepatocellular carcinoma mouse model. Interestingly, LA-PegPI/pluc nanoparticles could effectively stimulate the proliferation of NK cells and inhibit tumor growth in this model. In summary, LA-PegPI is a useful gene vector for immunogene therapy of hepatocellular carcinoma, and its potential for clinical application warrants further study. |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/13438]  |
| 专题 | 合成所 |
| 推荐引用方式 GB/T 7714 | Liu, Yi,Liu, Ling,Zong, Zhi-Min,et al. A novel galactose-PEG-conjugated biodegradable copolymer is an efficient gene delivery vector for immunotherapy of hepatocellular carcinoma[J]. BIOMATERIALS,2018. |
| APA | Liu, Yi.,Liu, Ling.,Zong, Zhi-Min.,Liu, Qian.,Jiang, Shuang-Shuang.,...&Yao, Hong.(2018).A novel galactose-PEG-conjugated biodegradable copolymer is an efficient gene delivery vector for immunotherapy of hepatocellular carcinoma.BIOMATERIALS. |
| MLA | Liu, Yi,et al."A novel galactose-PEG-conjugated biodegradable copolymer is an efficient gene delivery vector for immunotherapy of hepatocellular carcinoma".BIOMATERIALS (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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