Kif5b Modulates Processing and Secretion of von Willebrand Factor in Endothelial Cells and Mice
文献类型：期刊论文
| 作者 | Xingde Liu; Yu-Si Luo; Yue Zhuo; Ke Zhang; Erhard Hofer; Jian-Dong Huang |
| 刊名 | Cardiovascular Pharmacology
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Von Willebrand factor (VWF) secreted by Weibel-Palade bodies within endothelial cells is critical to maintain normal platelet adhesion during vascular arrest. Because the transportation of VWF is microtubule-dependent partly via kinesin activity, suppression of kinesin could affect intracellular traffcking of VWF. In this study, we investigated the role of Kif5b, the key member of the kinesin superfamily, in the processing and secretion of VWF. A hypothetical interaction between VWF and Kif5b was confrmed and the tail domain of Kif5b was identifed as VWF binding region. Knocking-down Kif5b in human umbilical vein endothelial cells led to signifcantly increased non-stimulated VWF secretion, considerably higher ratio of pro-VWF to mature VWF, and shorter VWF length. Consistent to the in vitro assay, Kif5b knockdown mice demonstrated dramatically increased VWF secretion after epinephrine stimulation. Signifcantly prolonged bleeding time was observed in these Kif5b knockdown mice as well, which was further elucidated by the decreased basal/regulated VWF secretion in Kif5b knockdown mice comparing to their wild-type littermates. Taken together, these fndings suggest that Kif5b modulates processing and secretion of VWF in vitro and in vivo |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/13457]  |
| 专题 | 合成所 |
| 推荐引用方式 GB/T 7714 | Xingde Liu,Yu-Si Luo,Yue Zhuo,et al. Kif5b Modulates Processing and Secretion of von Willebrand Factor in Endothelial Cells and Mice[J]. Cardiovascular Pharmacology,2018. |
| APA | Xingde Liu,Yu-Si Luo,Yue Zhuo,Ke Zhang,Erhard Hofer,&Jian-Dong Huang.(2018).Kif5b Modulates Processing and Secretion of von Willebrand Factor in Endothelial Cells and Mice.Cardiovascular Pharmacology. |
| MLA | Xingde Liu,et al."Kif5b Modulates Processing and Secretion of von Willebrand Factor in Endothelial Cells and Mice".Cardiovascular Pharmacology (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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