Nucleolar residence of the seckel syndrome protein TRAIP is coupled to ribosomal DNA transcription
文献类型：期刊论文
| 作者 | Cheng-han Yu; Yangzi Chen; Junshi Li; Fakun Cao; Jason Lam; Clooney CY Cheng; Michael SY Huen |
| 刊名 | Nucleic Acids Research
 |
| 出版日期 | 2018 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The RING finger protein TRAIP protects genome integrity and its mutation causes Seckel syndrome. TRAIP encodes a nucleolar protein that migrates to UV-induced DNA lesions via a direct interaction with the DNA replication clamp PCNA. Thus far, mechanistically how UV mobilizes TRAIP from the nucleoli remains unknown. We found that PCNA binding is dispensable for the nucleolus-nucleoplasm shuttling of TRAIP following cell exposure to UV irradiation, and that its redistribution did not rely on the master DNA damage kinases ATM and ATR. Interestingly, I-PpoI-induced ribosomal DNA damage led to TRAIP exclusion from the nucleoli, raising the possibility that active ribosomal DNA transcription may underlie TRAIP retention in the nuclear sub-compartments. Accordingly, chemical inhibition of RNA polymerase I activity led to TRAIP diffusion into the nucleoplasm, and was coupled with marked reduction of DNA/RNA hybrids in the nucleoli, suggesting that TRAIP may be sequestered via binding to nucleic acid structures in the nucleoli. Consistently, cell pre-treatment with DNase/RNase effectively released TRAIP from the nucleoli. Taken together, our study defines a bipartite mechanism that drives TRAIP trafficking in response to UV damage, and highlights the nucleolus as a stress sensor that contributes to orchestrating DNA damage responses. |
| 语种 | 英语 |
| 源URL | [http://ir.siat.ac.cn:8080/handle/172644/13461]  |
| 专题 | 合成所 |
| 推荐引用方式 GB/T 7714 | Cheng-han Yu,Yangzi Chen,Junshi Li,et al. Nucleolar residence of the seckel syndrome protein TRAIP is coupled to ribosomal DNA transcription[J]. Nucleic Acids Research,2018. |
| APA | Cheng-han Yu.,Yangzi Chen.,Junshi Li.,Fakun Cao.,Jason Lam.,...&Michael SY Huen.(2018).Nucleolar residence of the seckel syndrome protein TRAIP is coupled to ribosomal DNA transcription.Nucleic Acids Research. |
| MLA | Cheng-han Yu,et al."Nucleolar residence of the seckel syndrome protein TRAIP is coupled to ribosomal DNA transcription".Nucleic Acids Research (2018). |
入库方式： OAI收割
来源：深圳先进技术研究院
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