Ostrya chisosensis (Betulaceae) is a critically endangered species endemic to Texas. In this study, the complete chloroplast (cp) genome of O. chisosensis was assembled based on next generation sequencing. The cp genome was 159,107bp in length, including a large single copy (LSC) region of 88,458bp, a small single copy (SSC) region of 18,792bp and a pair of inverted repeats (IRs) of 51,857bp. The genome contained 124 genes, including 85 protein-coding genes, 30 tRNA genes and 8 ribosomal RNA genes. The majority of these gene species occurred as a single copy and 13 gene species occurred in two copies. The overall GC content of the chloroplast genome was 36.5%, whereas the corresponding values of the LSC, SSC, and IR regions were 34.3, 30.1 and 42.5%, respectively. A phylogenetic analysis demonstrated a close relationship between O. chisosensis and O. rehderiana.