CRISPRlnc: a manually curated database of validated sgRNAs for lncRNAs
文献类型:期刊论文
作者 | Chen, Wen1; Zhang, Guoqiang; Li, Jing; Zhang, Xuan; Huang, Shulan1; Xiang, Shuanglin1; Hu, Xiang1; Liu, Changning |
刊名 | NUCLEIC ACIDS RESEARCH |
出版日期 | 2019 |
卷号 | 47期号:D1页码:D63-D68 |
ISSN号 | 0305-1048 |
关键词 | Long Noncoding Rnas Activation Evolution Lincrnas Dna |
DOI | 10.1093/nar/gky904 |
英文摘要 | The CRISPR/Cas9 system, as a revolutionary genome editing tool for all areas of molecular biology, provides new opportunities for research on lncRNA's function. However, designing a CRISPR/Cas9 single guide RNA (sgRNA) for lncRNA is not easy with an unwarrantable effectiveness. Thus, it is worthy of collecting validated sgRNAs, to assist in efficiently choosing sgRNA with an expected activity. CRISPRlnc (http://www.crisprlnc.org or http://crisprlnc.xtbg.ac.cn) is a manually curated database of validated CRISPR/Cas9 sgRNAs for lncRNAs from all species. After manually reviewing more than 200 published literature, the current version of CRISPRlnc contains 305 lncRNAs and 2102 validated sgRNAs across eight species, including mammalian, insect and plant. We handled the ID, position in the genome, sequence and functional description of these lncRNAs, as well as the sequence, protoacceptor-motif (PAM), CRISPR type and validity of their paired sgRNAs. In CRISPRlnc, we provided the tools for browsing, searching and downloading data, as well as online BLAST service and genome browse server. As the first database against the validated sgRNAs of lncRNAs, CRISPRlnc will provide a new and powerful platform to promote CRISPR/Cas9 applications for future functional studies of lncRNAs. |
学科主题 | Biochemistry & Molecular Biology |
语种 | 英语 |
WOS记录号 | WOS:000462587400011 |
源URL | [http://ir.xtbg.org.cn/handle/353005/11221] |
专题 | 西双版纳热带植物园_2012年后新成立研究组 |
通讯作者 | Liu, Changning |
作者单位 | 1.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, Key Lab Trop Plant Resources & Sustainable Use, Kunming 650223, Yunnan, Peoples R China 2.Hunan Normal Univ, Sch Life Sci, State Key Lab Dev Biol Freshwater Fish, Changsha 410081, Hunan, Peoples R China |
推荐引用方式 GB/T 7714 | Chen, Wen,Zhang, Guoqiang,Li, Jing,et al. CRISPRlnc: a manually curated database of validated sgRNAs for lncRNAs[J]. NUCLEIC ACIDS RESEARCH,2019,47(D1):D63-D68. |
APA | Chen, Wen.,Zhang, Guoqiang.,Li, Jing.,Zhang, Xuan.,Huang, Shulan.,...&Liu, Changning.(2019).CRISPRlnc: a manually curated database of validated sgRNAs for lncRNAs.NUCLEIC ACIDS RESEARCH,47(D1),D63-D68. |
MLA | Chen, Wen,et al."CRISPRlnc: a manually curated database of validated sgRNAs for lncRNAs".NUCLEIC ACIDS RESEARCH 47.D1(2019):D63-D68. |
入库方式: OAI收割
来源:西双版纳热带植物园
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。