A systematic mass-transfer modeling approach for mammalian cell culture bioreactor scale-up
文献类型：期刊论文
| 作者 | He, Chuan1,2,4; Ye, Pei2; Wang, Haibin3; Liu, Xiao2; Li, Feng1,2 |
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2019-01-15 |
| 卷号 | 141页码:173-181 |
| 关键词 | Mammalian cell culture Mass-transfer model Bioreactor scale up |
| ISSN号 | 1369-703X |
| DOI | 10.1016/j.bej.2018.09.019 |
| 英文摘要 | A mathematical mass-transfer model was developed based on the mass balance of gas exchange in cell culture bioreactors. The model combines the bioreactor mass transfer properties and mammalian cell metabolic parameters to predict the cell culture process oxygen demands and carbon dioxide accumulation in bioreactors. With the guidance of the mass-transfer model, a CHO cell culture process to produce a therapeutic antibody was successfully scaled up from 2-L to 1500-L bioreactor directly without intermediate steps. The process performances and product quality attributes demonstrate good robustness and consistency between small and large scales. This mass-transfer model provides a more systematic and reliable approach for mammalian cell culture bioreactor scaling up. |
| WOS关键词 | DISSOLVED-OXYGEN ; ELEVATED PCO(2) ; GROWTH ; BICARBONATE ; OSMOLALITY ; DESIGN ; DAMAGE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000453623900018 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/27629]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Li, Feng |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China 2.Beijing Mabworks Biotech Co Ltd, Beijing 100176, Peoples R China 3.Zhejiang Hisun Pharmaceut Co Ltd, Hangzhou 311404, Zhejiang, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Chuan,Ye, Pei,Wang, Haibin,et al. A systematic mass-transfer modeling approach for mammalian cell culture bioreactor scale-up[J]. BIOCHEMICAL ENGINEERING JOURNAL,2019,141:173-181. |
| APA | He, Chuan,Ye, Pei,Wang, Haibin,Liu, Xiao,&Li, Feng.(2019).A systematic mass-transfer modeling approach for mammalian cell culture bioreactor scale-up.BIOCHEMICAL ENGINEERING JOURNAL,141,173-181. |
| MLA | He, Chuan,et al."A systematic mass-transfer modeling approach for mammalian cell culture bioreactor scale-up".BIOCHEMICAL ENGINEERING JOURNAL 141(2019):173-181. |
入库方式： OAI收割
来源：过程工程研究所
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