Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process
文献类型：期刊论文
| 作者 | Li, Wensong1; Yang, Liangrong2; Dong, Tingting2; Xing, Huifang2; Wang, Weiyan1; Yang, Yunquan1; Liu, Huizhou2 |
| 刊名 | AICHE JOURNAL
 |
| 出版日期 | 2019 |
| 卷号 | 65期号:1页码:175-183 |
| ISSN号 | 0001-1541 |
| 关键词 | gas-assisted magnetic separation large-scale bio-separation continuous low field separation of proteins |
| DOI | 10.1002/aic.16389 |
| 英文摘要 | In this study, the gas-assisted low-field magnetic separation (GAMS) was proposed for continuous large-scale magnetic bio-separation process. Magnetic recovery processes in selective separation of binary mixed proteins by magnetic microspheres were selected as a model. Basing on magnetic recovery efficiency of the batch GAMS for this model, the continuous GAMS with an amplifying small pilot scale was further researched. The continuous throughput of GAMS for adsorption, washing, and desorption step reached 12 L/h, 12 L/h, and 18 L/h with an adsorbent recovery above 95%, respectively. Furthermore, magnetic particles via the top magnetic roller could be well-separated from free proteins with good floatability via the bottom outlet of flotation column, and bubbling operation did not cause the significant denaturation of target protein. Compared with classic high gradient magnetic separation, GAMS had the advantages of easy scale-up, easy continuous operation, and low-cost, showing great potential for large-scale magnetic bio-separation process. (c) 2018 American Institute of Chemical Engineers AIChE J, 65: 175-183, 2019 |
| WOS关键词 | IRON-OXIDE NANOPARTICLES ; SUPERPARAMAGNETIC EXTRACTION ; PURIFICATION ; FLOTATION |
| 资助项目 | National Natural Science Foundation of China[21506180] ; National Natural Science Foundation of China[21676273] ; National Key Natural Science Foundation of China[U1507203] ; National Technology Research and Development Program of China[2015CB251402] ; Science and technology innovation project of CMST[17-163-12-ZD-001-013-01] ; Youth Innovation Promotion Association, CAS[2016043] ; Natural Science Foundation of Hunan Province, China[2016JJ3111] |
| WOS研究方向 | Engineering |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000454632100017 |
| 资助机构 | National Natural Science Foundation of China ; National Key Natural Science Foundation of China ; National Technology Research and Development Program of China ; Science and technology innovation project of CMST ; Youth Innovation Promotion Association, CAS ; Natural Science Foundation of Hunan Province, China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/27674]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Yang, Liangrong; Liu, Huizhou |
| 作者单位 | 1.Xiangtan Univ, Coll Chem Engn, Xiangtan, Hunan, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, CAS Key Lab Green Proc & Engn, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Wensong,Yang, Liangrong,Dong, Tingting,et al. Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process[J]. AICHE JOURNAL,2019,65(1):175-183. |
| APA | Li, Wensong.,Yang, Liangrong.,Dong, Tingting.,Xing, Huifang.,Wang, Weiyan.,...&Liu, Huizhou.(2019).Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process.AICHE JOURNAL,65(1),175-183. |
| MLA | Li, Wensong,et al."Gas-assisted low-field magnetic separation for large scale continuous magnetic bio-separation process".AICHE JOURNAL 65.1(2019):175-183. |
入库方式： OAI收割
来源：过程工程研究所
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