Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation
文献类型:期刊论文
作者 | Jia, Jinru1,2; Chen, Huayou1,3; Wu, Bangguo1; Cui, Fengjie1,4; Fang, Hua4; Wang, Hongcheng1; Ni, Zhong1 |
刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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出版日期 | 2019 |
卷号 | 187期号:1页码:253-265 |
关键词 | Neurospora crassa Multi-strain fermentation Solid-state fermentation True protein Rice straw |
ISSN号 | 0273-2289 |
DOI | 10.1007/s12010-018-2792-5 |
英文摘要 | Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of SSF, including the optimum inoculum size of mixed strains, inoculation ratio, and different inoculation time of N. crassa 14-8, were optimized. The experimental results showed that the highest TP content could be obtained by using N. crassa 14-8, C. utilis, and P. chrysosporium as mixed strains, and 5mM Mn2+ and 50mM veratryl alcohol were used as inducers of lignin peroxidase (LiP) to improve the efficiency of enzymatic hydrolysis. When N. crassa 14-8 was inoculated 1day later than P. chrysosporium, the total inoculum size was 10%, and the optimum ratio of N. crassa 14-8 to P. chrysosporium was 1:2, the maximum TP yield (8.89%) was obtained, with 123.37% of its increase rate. This work proposed a technique with potential application in large-scale feedstuff protein conversion. |
WOS关键词 | SOLID-STATE FERMENTATION ; SINGLE-CELL PROTEIN ; PHANEROCHAETE-CHRYSOSPORIUM ; FUNGAL PRETREATMENT ; NEUROSPORA-CRASSA ; DEGRADATION ; CELLULASE ; ENZYMES ; LACCASE ; ETHANOL |
资助项目 | Key R&D Program of Jiangsu Province (Modern Agriculture), China[BE2017355] ; Agricultural Sci-Tech Self-Innovation Program of Jiangsu Province, CX (17) 3044, China ; Open Funding Project of National Key Laboratory of Biochemical Engineering ; Jiangsu Special Research and Development Grant for Northern Jiangsu Area, China[SZ-YC2017001] |
WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
语种 | 英语 |
WOS记录号 | WOS:000455325800018 |
出版者 | HUMANA PRESS INC |
资助机构 | Key R&D Program of Jiangsu Province (Modern Agriculture), China ; Agricultural Sci-Tech Self-Innovation Program of Jiangsu Province, CX (17) 3044, China ; Open Funding Project of National Key Laboratory of Biochemical Engineering ; Jiangsu Special Research and Development Grant for Northern Jiangsu Area, China |
源URL | [http://ir.ipe.ac.cn/handle/122111/27746] ![]() |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Chen, Huayou |
作者单位 | 1.Jiangsu Univ, Inst Life Sci, Zhenjiang 212000, Peoples R China 2.Henan Prov Peoples Hosp, Translat Res Inst, Zhengzhou 450000, Henan, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 4.Jiangsu Yancheng Yuanyao Biotechnol Co Ltd, Yancheng 224000, Peoples R China |
推荐引用方式 GB/T 7714 | Jia, Jinru,Chen, Huayou,Wu, Bangguo,et al. Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2019,187(1):253-265. |
APA | Jia, Jinru.,Chen, Huayou.,Wu, Bangguo.,Cui, Fengjie.,Fang, Hua.,...&Ni, Zhong.(2019).Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,187(1),253-265. |
MLA | Jia, Jinru,et al."Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 187.1(2019):253-265. |
入库方式: OAI收割
来源:过程工程研究所
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