中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation

文献类型:期刊论文

作者Jia, Jinru1,2; Chen, Huayou1,3; Wu, Bangguo1; Cui, Fengjie1,4; Fang, Hua4; Wang, Hongcheng1; Ni, Zhong1
刊名APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
出版日期2019
卷号187期号:1页码:253-265
关键词Neurospora crassa Multi-strain fermentation Solid-state fermentation True protein Rice straw
ISSN号0273-2289
DOI10.1007/s12010-018-2792-5
英文摘要Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of SSF, including the optimum inoculum size of mixed strains, inoculation ratio, and different inoculation time of N. crassa 14-8, were optimized. The experimental results showed that the highest TP content could be obtained by using N. crassa 14-8, C. utilis, and P. chrysosporium as mixed strains, and 5mM Mn2+ and 50mM veratryl alcohol were used as inducers of lignin peroxidase (LiP) to improve the efficiency of enzymatic hydrolysis. When N. crassa 14-8 was inoculated 1day later than P. chrysosporium, the total inoculum size was 10%, and the optimum ratio of N. crassa 14-8 to P. chrysosporium was 1:2, the maximum TP yield (8.89%) was obtained, with 123.37% of its increase rate. This work proposed a technique with potential application in large-scale feedstuff protein conversion.
WOS关键词SOLID-STATE FERMENTATION ; SINGLE-CELL PROTEIN ; PHANEROCHAETE-CHRYSOSPORIUM ; FUNGAL PRETREATMENT ; NEUROSPORA-CRASSA ; DEGRADATION ; CELLULASE ; ENZYMES ; LACCASE ; ETHANOL
资助项目Key R&D Program of Jiangsu Province (Modern Agriculture), China[BE2017355] ; Agricultural Sci-Tech Self-Innovation Program of Jiangsu Province, CX (17) 3044, China ; Open Funding Project of National Key Laboratory of Biochemical Engineering ; Jiangsu Special Research and Development Grant for Northern Jiangsu Area, China[SZ-YC2017001]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000455325800018
出版者HUMANA PRESS INC
资助机构Key R&D Program of Jiangsu Province (Modern Agriculture), China ; Agricultural Sci-Tech Self-Innovation Program of Jiangsu Province, CX (17) 3044, China ; Open Funding Project of National Key Laboratory of Biochemical Engineering ; Jiangsu Special Research and Development Grant for Northern Jiangsu Area, China
源URL[http://ir.ipe.ac.cn/handle/122111/27746]  
专题中国科学院过程工程研究所
通讯作者Chen, Huayou
作者单位1.Jiangsu Univ, Inst Life Sci, Zhenjiang 212000, Peoples R China
2.Henan Prov Peoples Hosp, Translat Res Inst, Zhengzhou 450000, Henan, Peoples R China
3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China
4.Jiangsu Yancheng Yuanyao Biotechnol Co Ltd, Yancheng 224000, Peoples R China
推荐引用方式
GB/T 7714
Jia, Jinru,Chen, Huayou,Wu, Bangguo,et al. Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2019,187(1):253-265.
APA Jia, Jinru.,Chen, Huayou.,Wu, Bangguo.,Cui, Fengjie.,Fang, Hua.,...&Ni, Zhong.(2019).Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,187(1),253-265.
MLA Jia, Jinru,et al."Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 187.1(2019):253-265.

入库方式: OAI收割

来源:过程工程研究所

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