Effect of Pore Structure on Protein Capacity in Macroporous Polymer Chromatographic Supports
文献类型:期刊论文
作者 | Li Heng1; Wang Shaoyun2; Fang Jiaxuan1; Zhao Lan3; Jin Haibo1; He Guangxiang1; Guo Xiaoyan1; Gu Qingyang1; Hao Siwen1; Re Ziya1 |
刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE |
出版日期 | 2018-12-10 |
卷号 | 39期号:12页码:2673-2678 |
ISSN号 | 0251-0790 |
关键词 | Macroporous Polymer Microsphere Chromatographic Media Protein Capactiy High Through-put |
DOI | 10.7503/cjcu20180304 |
英文摘要 | Macroporous microspheres were prepared through suspension polymerization, based on a copolymer of glycidyl methacrylate and ethylene glycol dimethacrylate , which were used for functional monomer and crosslinking agent, respectively. The effect of porogen on microspheres structure was evaluated in terms of pore size and surface area. The anion exchanged supports were prepared through derivation of microspheres with poly ( ethylene imine). The relation of the microspheres structure and the protein capacity was examined on these anion exchanged media. The results indicated that the pore size of microspheres increased with the poor solvent in the porogen ( good solvent/poor solvent = 1 : 1-1 : 3. 5 ) , however, the surface area showed a contrary trend. The ion exchanged capacity ( 0. 11-0. 27 mmol/mL) increased with the surface area of the microspheres (4-38 m(2)/g) , and the responding static binding capacity of proteins also show a positive correlation with the surface area. The dynamic binding capacity of proteins firstly increased and then retained a changeless value in the pore range of 301-1524 nm. This value was retained at 13 mg/mL when the pore size was more than 410 nm. It indicated that the surface area could not influence the dynamic binding capacity while the pore size of media was beyond some value. Furthermore, the large biomolecular transport in the microspheres was observed through laser scanning confocal microscopy. The results indicated that hepatitis B virus surface antigen ( HBsAg) could enter freely the microsphere with all of above pore size. The above results provide a reference for fabrication of the chromatographic supports. |
WOS关键词 | Virus-like Particles ; Media ; Microspheres ; Adsorption |
资助项目 | Project of Beijing Natural Science Foundation, China[2162013] ; Project of Beijing Natural Science Foundation, China[2172054] ; Science and Technology Projects of Beijing Education Commission, China[KM201710017002] ; Project of Support Plan for the Construction of High Level Teachers and High-Level Innovation Team Building of Beijing Municipal University, China[IDHT20180508] ; Subject Platform Construction Project, China[2018XK002] ; 2018BIPT-SPBYSJ of China[18032021002] |
WOS研究方向 | Chemistry |
语种 | 英语 |
出版者 | HIGHER EDUCATION PRESS |
WOS记录号 | WOS:000456959800009 |
资助机构 | Project of Beijing Natural Science Foundation, China ; Science and Technology Projects of Beijing Education Commission, China ; Project of Support Plan for the Construction of High Level Teachers and High-Level Innovation Team Building of Beijing Municipal University, China ; Subject Platform Construction Project, China ; 2018BIPT-SPBYSJ of China |
源URL | [http://ir.ipe.ac.cn/handle/122111/27755] |
专题 | 中国科学院过程工程研究所 |
通讯作者 | Zhang Rongyue |
作者单位 | 1.Beijing Inst Petrochem Technol, Beijing Key Lab Fuels Cleaning & Adv Catalyt Emis, Beijing 102617, Peoples R China 2.Senhui Microsphere Tech Suzhou Co Ltd, Suzhou 215123, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Li Heng,Wang Shaoyun,Fang Jiaxuan,et al. Effect of Pore Structure on Protein Capacity in Macroporous Polymer Chromatographic Supports[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2018,39(12):2673-2678. |
APA | Li Heng.,Wang Shaoyun.,Fang Jiaxuan.,Zhao Lan.,Jin Haibo.,...&Zhang Rongyue.(2018).Effect of Pore Structure on Protein Capacity in Macroporous Polymer Chromatographic Supports.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,39(12),2673-2678. |
MLA | Li Heng,et al."Effect of Pore Structure on Protein Capacity in Macroporous Polymer Chromatographic Supports".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 39.12(2018):2673-2678. |
入库方式: OAI收割
来源:过程工程研究所
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