中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 过程工程研究所
  3. 中国科学院过程工程研究所
Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms

文献类型：期刊论文

作者Li, Ruilian1,2; Yuan, Xianghua3; Wei, Jinhua2; Zhang, Xiafei3; Cheng, Gong2; Wang, Zhuo A.2; Du, Yuguang2
刊名MARINE DRUGS
出版日期2019
卷号17期号:1页码:12
关键词chitosan oligosaccharides streptomycin Pseudomonas aeruginosa biofilms conjugation
ISSN号1660-3397
DOI10.3390/md17010043
英文摘要Microbial biofilms are considerably more resistant to antibiotics than planktonic cells. It has been reported that chitosan coupling with the aminoglycoside antibiotic streptomycin dramatically disrupted biofilms of several Gram-positive bacteria. This finding suggested the application of the covalent conjugate of antimicrobial natural polysaccharides and antibiotics on anti-infection therapy. However, the underlying molecular mechanism of the chitosan-streptomycin conjugate (CS-Strep) remains unclear and the poor water-solubility of the conjugate might restrict its applications for anti-infection therapy. In this study, we conjugated streptomycin with water-soluble chitosan oligosaccharides (COS). Unlike CS-Strep, the COS-streptomycin conjugate (COS-Strep) barely affected biofilms of tested Gram-positive bacteria. However, COS-Strep efficiently eradicated established biofilms of the Gram-negative pathogen Pseudomonas aeruginosa. This activity of COS-Strep was influenced by the degree of polymerization of chitosan oligosaccharide. The increased susceptibility of P. aeruginosa biofilms to antibiotics after conjugating might be related to the following: Suppression of the activation of MexX-MexY drug efflux pump system induced by streptomycin treatment; and down-regulation of the biosynthesis of biofilm exopolysaccharides. Thus, this work indicated that covalently linking antibiotics to chitosan oligosaccharides was a possible approach for the development of antimicrobial drugs against biofilm-related infections.
WOS关键词ANTIBACTERIAL ACTIVITY ; ANTIBIOTIC-RESISTANCE ; PHARMACOKINETICS/PHARMACODYNAMICS ; COLISTIN ; IMIPENEM ; ASSAY
资助项目National Natural Science Fund, China[31670809] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-218] ; Open Project Program of the Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University[BM2016004]
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000458053200043
出版者MDPI
资助机构National Natural Science Fund, China ; Key Research Program of the Chinese Academy of Sciences ; Open Project Program of the Collaborative Innovation Center of Modern Bio-Manufacture, Anhui University
源URL[http://ir.ipe.ac.cn/handle/122111/27828]  
专题中国科学院过程工程研究所
通讯作者Wang, Zhuo A.; Du, Yuguang
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, PLA & State Key Lab Biochem Engn, Key Lab Biopharmaceut Prod & Formulat Engn, Beijing 100190, Peoples R China
3.Sichuan Normal Univ, Coll Life Sci, Chengdu 610101, Sichuan, Peoples R China
推荐引用方式
GB/T 7714		 Li, Ruilian,Yuan, Xianghua,Wei, Jinhua,et al. Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms[J]. MARINE DRUGS,2019,17(1):12.
APA Li, Ruilian.,Yuan, Xianghua.,Wei, Jinhua.,Zhang, Xiafei.,Cheng, Gong.,...&Du, Yuguang.(2019).Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms.MARINE DRUGS,17(1),12.
MLA Li, Ruilian,et al."Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms".MARINE DRUGS 17.1(2019):12.

入库方式： OAI收割

来源：过程工程研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。