Ammonium Nitrogen Tolerant Chlorella Strain Screening and Its Damaging Effects on Photosynthesis
文献类型：期刊论文
| 作者 | Wang, Qiang2,3; He, Chenliu3; Zhan, Jiao3; Chen, Hui1,3; Zhou, Wei2,3; Wang, Jie2,3 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2019-01-07 |
| 卷号 | 9页码:13 |
| 关键词 | ammonium nitrogen biological remediation Chlorella oxygen-evolving complex photosynthesis |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2018.03250 |
| 英文摘要 | Nitrogen is an essential nutrient element. Ammonium nitrogen, one of the most common nitrogen sources, is found in various habitats, especially wastewater. However, excessive amounts of ammonium nitrogen can be toxic to phytoplankton, higher plants, fish, and other animals, and microorganisms. In this study, we explored the tolerance of green algae to ammonium nitrogen using 10 Chlorella strains. High concentrations of ammonium nitrogen directly inhibited the growth of Chlorella, but the degree of inhibition varied by strain. With the EC50 of 1.6 and 0.4 g L-1, FACHB-1563 and FACHB-1216, respectively had the highest and lowest tolerance to ammonium nitrogen among all strains tested, suggesting that FACHB-1563 could potentially be used to remove excess ammonium nitrogen from wastewater in bioremediation efforts. Two strains with the highest and lowest tolerance to ammonium nitrogen were selected to further explore the inhibitory effect of ammonium nitrogen on Chlorella. Analysis of chlorophyll fluorescence, oxygen evolution, and photosynthesis proteins via immunoblot showed that photosystem II (PSII) had been damaged when exposed to high levels of ammonium nitrogen, with the oxygen-evolving complex as the primary site, and electron transport from Q(A)(-) to Q(B) was subsequently inhibited by this treatment. A working model of ammonium nitrogen competition between N assimilation and PSII damage is proposed to elucidate that the assimilation rate of ammonium nitrogen by algae strains determines the tolerance of cells to ammonium nitrogen toxicity. |
| 资助项目 | National Natural Science Foundation of China[31870041] ; National Natural Science Foundation of China[31770128] ; National Natural Science Foundation of China[31700107] ; Hubei Provincial Natural Science Foundation of China[2017CFA021] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FB11] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000455004600001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6281]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, Qiang |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Qiang,He, Chenliu,Zhan, Jiao,et al. Ammonium Nitrogen Tolerant Chlorella Strain Screening and Its Damaging Effects on Photosynthesis[J]. FRONTIERS IN MICROBIOLOGY,2019,9:13. |
| APA | Wang, Qiang,He, Chenliu,Zhan, Jiao,Chen, Hui,Zhou, Wei,&Wang, Jie.(2019).Ammonium Nitrogen Tolerant Chlorella Strain Screening and Its Damaging Effects on Photosynthesis.FRONTIERS IN MICROBIOLOGY,9,13. |
| MLA | Wang, Qiang,et al."Ammonium Nitrogen Tolerant Chlorella Strain Screening and Its Damaging Effects on Photosynthesis".FRONTIERS IN MICROBIOLOGY 9(2019):13. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。