Encapsulating Quantum Dots within HIV-1 Virions through Site-Specific Decoration of the Matrix Protein Enables Single Virus Tracking in Live Primary Macrophages
文献类型：期刊论文
| 作者 | Cui, Zongqiang3; Zhang, Xian-En2; Zhang, Xiaowei3; Zhang, Zhiping3; Li, Wei3; Yin, Wen3; Li, Qin1,3 |
| 刊名 | NANO LETTERS
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 18期号:12页码:7457-7468 |
| 关键词 | Quantum dots HIV-1 matrix single-particle tracking mitochondria fission |
| ISSN号 | 1530-6984 |
| DOI | 10.1021/acs.nanolett.8b02800 |
| 英文摘要 | Labeling and imaging with quantum dots (QDs) provides powerful tools to visualize viral infection in living cells. Encapsulating QDs within virions represents a novel strategy for virus labeling. Here, we developed infectious HIV-1 virions encapsulating QDs through site-specific decoration of the viral matrix protein (MA) and used them to visualize early infection events in human primary macrophages by single-particle imaging. The MA protein was fused to a biotin acceptor peptide (BAP) tag, biotinylated, complexed with streptavidin-conjugated QDs in live cells, and incorporated into virions during virus assembly. The QD-encapsulated virions were tracked during infection of macrophages at a single particle level. The dynamic dissociation of MA and Vpr was also tracked in real time, and the results demonstrated that MA has multiple dynamic behaviors and functions during virus entry. More importantly, we tracked the dynamic interplay of QD-encapsulated virions with cellular mitochondria in live primary macrophages. We also found that HIV-1 can induce fission of mitochondria during the early phases of infection. In summary, we have constructed a type of QD-encapsulated virus particle and used this technology to further our understanding of the early events of HIV-1 infection. |
| 资助项目 | National Key Research and Development Program of China[2018ZX10301405] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB29050000] ; National Natural Science Foundation of China[31470269] ; National Natural Science Foundation of China[21727816] ; National Natural Science Foundation of China[31470837] ; Youth Innovation Promotion Association of Chinese Academy of Sciences |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000453488800006 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6293]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Cui, Zongqiang |
| 作者单位 | 1.Fujian Normal Univ, Coll Life Sci, Engn Res Ctr Ind Microbiol, Minist Educ, Fuzhou 350007, Fujian, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Zongqiang,Zhang, Xian-En,Zhang, Xiaowei,et al. Encapsulating Quantum Dots within HIV-1 Virions through Site-Specific Decoration of the Matrix Protein Enables Single Virus Tracking in Live Primary Macrophages[J]. NANO LETTERS,2018,18(12):7457-7468. |
| APA | Cui, Zongqiang.,Zhang, Xian-En.,Zhang, Xiaowei.,Zhang, Zhiping.,Li, Wei.,...&Li, Qin.(2018).Encapsulating Quantum Dots within HIV-1 Virions through Site-Specific Decoration of the Matrix Protein Enables Single Virus Tracking in Live Primary Macrophages.NANO LETTERS,18(12),7457-7468. |
| MLA | Cui, Zongqiang,et al."Encapsulating Quantum Dots within HIV-1 Virions through Site-Specific Decoration of the Matrix Protein Enables Single Virus Tracking in Live Primary Macrophages".NANO LETTERS 18.12(2018):7457-7468. |
入库方式： OAI收割
来源：武汉植物园
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