The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM
文献类型：期刊论文
| 作者 | Francis, Matthew S.7,8; Dersch, Petra3; Chen, Shiyun4; Thompson, Karl M.5,6; Obi, Ikenna R.1,7,8; Francis, Monika K.7,8; Ruuth, Kristina7,8; Gurung, Jyoti M.7,8; Sundbom, Marcus Fredriksson8; Liu, Junfa2,7,8 |
| 刊名 | VIRULENCE
 |
| 出版日期 | 2019 |
| 卷号 | 10期号:1页码:37-57 |
| 关键词 | Environmental stress responsiveness gene expression control metabolic networks microbial behaviour growth and survival fitness |
| ISSN号 | 2150-5594 |
| DOI | 10.1080/21505594.2018.1556151 |
| 英文摘要 | The Gram-negative enteropathogen Yersinia pseudotuberculosis possesses a number of regulatory systems that detect cell envelope damage caused by noxious extracytoplasmic stresses. The CpxA sensor kinase and CpxR response regulator two-component regulatory system is one such pathway. Active Cpx signalling upregulates various factors designed to repair and restore cell envelope integrity. Concomitantly, this pathway also down-regulates key determinants of virulence. In Yersinia, cpxA deletion accumulates high levels of phosphorylated CpxR (CpxR similar to P). Accumulated CpxR similar to P directly repressed rovA expression and this limited expression of virulence-associated processes. A second transcriptional regulator, RovM, also negatively regulates rovA expression in response to nutrient stress. Hence, this study aimed to determine if CpxR similar to P can influence rovA expression through control of RovM levels. We determined that the active CpxR similar to P isoform bound to the promoter of rovM and directly induced its expression, which naturally associated with a concurrent reduction in rovA expression. Site-directed mutagenesis of the CpxR similar to P binding sequence in the rovM promoter region desensitised rovM expression to CpxR similar to P. These data suggest that accumulated CpxR similar to P inversely manipulates the levels of two global transcriptional regulators, RovA and RovM, and this would be expected to have considerable influence on Yersinia pathophysiology and metabolism. |
| 资助项目 | Swedish Research Council (Vetenskapsradet)[2009-3660] ; Swedish Research Council (Vetenskapsradet)[2014-6652] ; Foundation for Medical Research at Umea University ; Faculty of Science and Technology at Umea University |
| WOS研究方向 | Immunology ; Infectious Diseases ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000453473300001 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6298]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Francis, Matthew S. |
| 作者单位 | 1.Umea Univ, Dept Med Biochem & Biophys, SE-90187 Umea, Sweden 2.Guangdong Wens Foodstuffs Grp Co Ltd, Res Inst, Yunfu 527400, Guangdong, Peoples R China 3.Helmholtz Ctr Infect Res, Dept Mol Infect Biol, Braunschweig, Germany 4.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 5.Howard Univ, Interdisciplinary Res Bldg, Washington, DC 20059 USA 6.Howard Univ, Coll Med, Dept Microbiol, Washington, DC USA 7.Umea Univ, Umea Ctr Microbial Res, Umea, Sweden 8.Umea Univ, Dept Mol Biol, Umea, Sweden |
| 推荐引用方式 GB/T 7714 | Francis, Matthew S.,Dersch, Petra,Chen, Shiyun,et al. The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM[J]. VIRULENCE,2019,10(1):37-57. |
| APA | Francis, Matthew S..,Dersch, Petra.,Chen, Shiyun.,Thompson, Karl M..,Obi, Ikenna R..,...&Thanikkal, Edvin J..(2019).The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM.VIRULENCE,10(1),37-57. |
| MLA | Francis, Matthew S.,et al."The Yersinia pseudotuberculosis Cpx envelope stress system contributes to transcriptional activation of rovM".VIRULENCE 10.1(2019):37-57. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。