Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles
文献类型：期刊论文
| 作者 | Zhou, Yuan3; Wu, Chunchen3; Chen, Xinwen3; Hu, Xue3; Chen, Jizheng3; Chen, Yingshan3; Cao, Liang2,3; Deng, Liu1,2,3 |
| 刊名 | FUTURE VIROLOGY
 |
| 出版日期 | 2018-11-01 |
| 卷号 | 13期号:11页码:769-778 |
| 关键词 | fluorescent protein hepatitis B surface antigen imaging mCherry signal peptide subviral particles |
| ISSN号 | 1746-0794 |
| DOI | 10.2217/fvl-2018-0099 |
| 英文摘要 | Aim: To label HBsAg with the mCherry protein without impairing its functionality. Materials & methods: A vector expressing mCherry-HBsAg fusion protein was constructed and transfected into Huh7 cell lines. The expression, secretion and subcellular localization of HBsAg was detected by western blotting, ELISA and immunofluorescence staining, respectively. Then the assembly of subviral particles was evaluated by sucrose density gradient centrifugation, dot blotting and electron microscopic assay. Results: mCherry-HBsAg fusion protein can be expressed and secreted in a similar manner to HBsAg. More importantly, mCherry-HBsAg fusion protein can self-assemble into spherical subviral-like particles. Conclusion: mCherry could be introduced into HBsAg without affecting its biological characters including expression, secretion and assembly. |
| 资助项目 | National Nature Science Foundation of China[31770180] ; Youth Innovation Promotion Association CAS[2016303] |
| WOS研究方向 | Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:000452398000003 |
| 出版者 | FUTURE MEDICINE LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6330]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhou, Yuan |
| 作者单位 | 1.NU, Feinberg Sch Med, Dept Microbiol & Immunol, Chicago, IL USA 2.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yuan,Wu, Chunchen,Chen, Xinwen,et al. Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles[J]. FUTURE VIROLOGY,2018,13(11):769-778. |
| APA | Zhou, Yuan.,Wu, Chunchen.,Chen, Xinwen.,Hu, Xue.,Chen, Jizheng.,...&Deng, Liu.(2018).Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles.FUTURE VIROLOGY,13(11),769-778. |
| MLA | Zhou, Yuan,et al."Hepatitis B surface antigen with N-terminal addition of mCherry can assemble into functional subviral particles".FUTURE VIROLOGY 13.11(2018):769-778. |
入库方式： OAI收割
来源：武汉植物园
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