Outcrossing Rates and Gene Flow in Natural Population of the Endangered Endemic Aquatic Lycophyte Isoetes yunguiensis as Revealed by ISSR Markers
文献类型：期刊论文
| 作者 | Liu, Hong3; Qin, Rui3; Hao, Fei1; Liao, Kuo2; Dong, Xiang3; Ma, Mengxue3 |
| 刊名 | NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
 |
| 出版日期 | 2019 |
| 卷号 | 47期号:2页码:339-346 |
| 关键词 | endangered genetic structure ISSR Isoetes yunguiensis outcrossing rate |
| ISSN号 | 0255-965X |
| DOI | 10.15835/nbha47211264 |
| 英文摘要 | In this study, mating system, genetic diversity, and genetic structure of the endangered endemic aquatic Isoetes yunguiensis in China was investigated using ISSR markers. The results of ISSR analyses showed that the estimate of multilocus outcrossing rate (t(m)) was high at species level (t(m) = 0.955), indicating that diploid I. yunguiensis is a predominant outcrossing species. Nine selected ISSR primers used in the study amplified 66 reproducible bands, 41 of which were polymorphic among 37 individuals. High level of genetic diversity was detected at the species level (PPB = 62.12%), whereas, relatively low genetic diversity existed within populations (PPB = 39.39%). Analysis of molecular variance (AMOVA) revealed that 31.99% of the genetic variation was attributable to differences between populations and the rest (68.01%) to variability within populationsof I. yunguiensis. Value of F-st (0.320) indicated that genetic differentiation between populations also was significant. These results showed that I. yunguiensis predominantly favors crossing, and has a high level of genetic diversity and highly significant genetic variation between and within populations. Gene flow (Nm) among populations is equal to 1.177. High outcrossing rates may be responsible for the high levels of genetic diversity observed in the I. yunguiensis population. To maintain the current level of genetic diversity for this species, we recommend increasing in situ conservation sites. |
| 资助项目 | National Natural Science Foundation of China[31170341] ; National Natural Science Foundation of China[31200170] ; National Species Fund on Basic Research of Science and Technology of China[2014FY110100] ; Construction Plan of Hubei Province Science and Technology basic conditions platform[2017BEC014] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000454169000010 |
| 出版者 | UNIV AGR SCI & VETERINARY MED CLUJ-NAPOCA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6431]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Liu, Hong; Qin, Rui |
| 作者单位 | 1.Northwestern Polytech Univ, Coll Life Sci, Xian 710129, Shaanxi, Peoples R China 2.Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 3.South Cent Univ Nationalities, Key Lab Protect & Applicat Special Plant Germplas, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Hong,Qin, Rui,Hao, Fei,et al. Outcrossing Rates and Gene Flow in Natural Population of the Endangered Endemic Aquatic Lycophyte Isoetes yunguiensis as Revealed by ISSR Markers[J]. NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA,2019,47(2):339-346. |
| APA | Liu, Hong,Qin, Rui,Hao, Fei,Liao, Kuo,Dong, Xiang,&Ma, Mengxue.(2019).Outcrossing Rates and Gene Flow in Natural Population of the Endangered Endemic Aquatic Lycophyte Isoetes yunguiensis as Revealed by ISSR Markers.NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA,47(2),339-346. |
| MLA | Liu, Hong,et al."Outcrossing Rates and Gene Flow in Natural Population of the Endangered Endemic Aquatic Lycophyte Isoetes yunguiensis as Revealed by ISSR Markers".NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA 47.2(2019):339-346. |
入库方式： OAI收割
来源：武汉植物园
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